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作 者 ：徐桂芳 张静兰

期 刊 ：植物学通报 1991年 8卷 02期 页码：34-38

关键词：绿豆子叶；愈伤组织；RNA聚合酶；a-鹅膏菌素；

摘 要 ：绿豆(Phaseolus vadiatus L.)子叶切段在脱分化形成愈伤组织过程中，制备的染色质具有较高的RNA聚合酶活力，3天愈伤组织的酶活力高于5天和7天的；3天、5天和7天加激素脱柱依次分分化形成的愈伤组织，其酶活力均比不脱分化的高一倍以上， 用DEAE —纤维素酶部，依据层析洗脱性质和层析图谱及对d—鹅膏菌素的敏感程度，证明三个峰是RNA聚合I，II和III。

Abstract:Chromtin bound DNA-dependent RNA polymerase was isolated from mung bean cotyledon segment during dedifferentiation. The results show that the activity of RNA polymerase in dedifferentiation tissue after culturing for 3 days is higher than that of 5 and 7 days. The activities of RNA polymerase in dedifferentiation tissue for 3, 5 and 7 days are higher than that of control. After fractionation on DEAE-CELLUOSE column, three enzyme peaks were detected in the elute fractions. According to their sensitivity to a-amanitin they are the RNA polymerases I, II and III resp.

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