全 文 ：Effects of Water Extract from Datura fastuosa L． and
Chelidonium majus L． on Sterilization
SONG Rui-jia* ，HOU Wei-zhuo，YANG Jie，FENG Jing，ZHANG Xiao-na，FAN Hong-zhe
Chengde Medical College，Chende 067000，China
Abstract ［Objective］This paper is to learn the effects of Datura fastuosa L． and Chelidonium majus L． on sterilization by studying their effect on
S． aureus and E． coli． ［Method］This study selected the ripe under yearling Datura fastuosa L． and Chelidonium majus L． with boiling water，re-
spectively，by taking Shuang Huang Lian as the control． And the effect of Datura fastuosa L． and Chelidonium majus L． on S． aureus and E． coli
was also studied． ［Result］There was no effect of Datura fastuosa L． on sterilization． There was effect of Chelidonium majus L． on sterilization，but
there was more effect of masculine S． aureus and E． coli． ［Conclusion］Chelidonium majus L． can kill S． aureus and E． coli． But Datura fastuosa
L． can not kill the S． aureus and E． coli．
Key words Datura fastuosa L．，Chelidonium majus L．，Water extract，Sterilization，E． coli，S． aureus，China
Received:December 2，2009 Accepted:January 6，2010
Supported by the Students Research Program of Chengde Medical
College．
* Corresponding author． E-mail:zhenkon． g@163． com
Datura fastuosa L．，known as alias Yangjinhua，Gou-
hetao，Shandamazi et al，is an annual herb of the family So-
lanaceae． Its plants are poisonous，fruits are highly toxic;the
main active ingredients in Datura fastuosa L． are hyoscine，hyo-
scyamine et al． It can dissolve in water，and is slightly soluble
in alcohol;It has effects of suppressing cough and relieving
dyspnea，expelling wind，numbness and pain relief，sedation
and other effects in human clinical application［1］． Chelidonium
majus L．，known as Shanhuanglian，Babujin，Xionghuangcao
et al，is a perennial herb of the family Papaveraceae;the main
active ingredients are chelidonine，chelerythrine，coptisine et
al;it is toxic，cool in nature and bitter in flavor，having effects of
analgesic， suppressing cough， detoxification， detumes-
cence［1］． In the medicinal plants，poisonous plants play a sig-
nificant role in the course of the human production and life his-
tory． According to the utilization of resources，poisonous plants
are a kind of economic crops and belong to applied plant re-
sources． Only when recognizing them，development and utiliza-
tion of them can play its proper value． In order to develop and
apply toxic plant resources，we used silkworm as test material
to study contact poison and stomach poison actions of Datura
fastuosa L． aqueous extract to Lepidoptera． The results
showed that Datura fastuosa L． aqueous extract had no contact
poison for silkworm，but had significant anesthetic action［2］． On
this basis，we studied on the bactericidal effect of Datura fastu-
osa L． and Chelidonium majus L． aqueous extract，so as to lay
the foundation for the application of medicinal plants of agricul-
ture and Chinese medicine in the future．
1 Materials and methods
1．1 Materials
1． 1． 1 Tested strains． Escherichia coli standard strains，Staphy-
lococcus aureus standard strains．
1． 1． 2 Tested plants． Annual Datura fastuosa L． fresh plant
and annual Chelidonium majus L． complete stool．
1． 1． 3 Control drug． Shuang Huang Lian．
1． 2 Methods
1． 2． 1 Cultured strain． Add bacteria to nutrient broth and put it
in 37 ℃ constant temperature incubator for 18 －24 h． Dilute the
test bacteria by 100 fold as test bacteria liquid for future use．
Bacteria culture，etc． are all carried out in a sterile room．
1． 2． 2 Aqueous extract preparation． Use annual Datura fastu-
osa L． plant (including branches，leaves，flowers and fruits)
and annual Chelidonium majus L． plant at a appropriate quanti-
ty;extract with boiling water to make aqueous extract for
test［2］．
1． 2． 3 Liquid sterilization test． The test is carried out in 24 ori-
fices flats． One 24 orifices flat is taken as one group，contai-
ning three medicine samples of Datura fastuosa L．，Chelidoni-
um majus L． and Shuang Huang Lian，numbering asⅠ，Ⅱ，Ⅲ，
respectively． Each tested samples set five treatments (raw liq-
uor，1∶1 dilution，1∶2 dilution，1∶4 dilution，1∶8 dilution)． In ad-
dition，set positive control(Nutrient broth are added with bacte-
rial liquid only，without sterilization liquid)and negative control
(Nutrient broth are neither added with bacterial liquid nor sterili-
zation liquid)．
In 24 orifices flat，each medicine sample takes up one line
and five holes． Firstly，adding 1． 0 ml test medicine liquid to the
first hole and put 1． 0 ml nutrient broth in other 4 holes，respec-
tively． Then，add 1． 0 ml test medicine liquid to second hole
and make it into a 1∶1 dilution． 1． 0 ml liquid from second hole is
taken to the third hole and make it into a dilution at 1∶2． Use the
same method to make the fourth，the fifth hole become 1∶4 dilu-
tion and 1∶8 dilution respectively． Take out the excrescent 1． 0
ml liquid from the last hole to make the same liquid volume in
each hole． Put 50 μl Escherichia coli in 1 －5 holes of each test
line on 24 orifices flat respectively． Take the sixth hole set as
positive control and negative control． Besides，another 24 ori-
Medicinal Plant /药用植物研究 2010，1(2):27 －28，32 Responsible editor:Jiang Li Responsible translator:Cheng Na
fices flat，according to the above operation，are added with
Staphylococcus aureus for test． Each treatment group set 2
repetitions．
Put the 24 orifices flat with test bacterium in 37 ℃ constant
temperature incubator for 18 －24 h，then take out and observe
the growth of bacteria in each hole． Turbidity means bacteria
growth，recording as + ，clear means no bacteria growth，re-
cording as － ，and clear with little turbidity is recorded as
± ． Therefore，it can judge whether the medicine liquid in the
hole of each treatment has anti-killing effect on bacteria or not［3］．
1． 2． 4 Plate culture observations． Join nutrient agar in 7． 5 mm
diameter petri-dish and make solid plate medium． Take 100 ml
Bacilli from each hole (1，2，and 3 respectively)by auto dis-
penser and put in solid plate medium，coating uniformity by
slide glass． Put it in the two flats for two times as two repli-
cates． Put the plate with Bacilli in 37 ℃ constant temperature
incubator for 18 －24 h;then take out and observe the growth of
bacteria in each plate． No bacterial growth is recorded as
○，less growth is recorded as colony and more growth is
recorded as lawn ．
2 Results and analysis
2．1 Test results of liquid sterilization It can be seen from
Table 1 that Datura fastuosa L． has no inhibitory or killing effect
on Escherichia coli and Staphylococcus aureus;Chelidonium
majus L． has significant inhibitory or killing effect on Escherichia
coli and Staphylococcus aureus，but the bactericidal effect is
weaker compared with the control (Shuang Huang Lian)．
Table 1 Results of the liquid bacterial culture in 24 orifices flat
Bacteria
Water
extract
No．
Raw
liquor
1∶1
dilution
1∶2
dilution
1∶4
dilution
1∶8
dilution
Negative
control
Positive
control
S． aureus D． fastuosa Ⅰ + + + + + _ +
C． majus Ⅱ － － + + + _ +
Shuang Huang Lian Ⅲ － － － ± + _ +
E． coli D． fastuosa Ⅰ + + + + + _ +
C． majus Ⅱ － + + + + _ +
Shuang Huang Lian Ⅲ － － ± + + _ +
2． 2 Plate culture observations According to Table 2，Da-
tura fastuosa L． has no inhibitory or killing effect on Escherichia
coli and Staphylococcus aureus，so that the plate is covered
with bacterial lawn; however， Chelidonium majus L． and
Shuang Huang Lian has more anti-killing effect on Staphylococ-
cus aureus than Escherichia coli．
Table 2 Results of bacterial culture in flat plate
Bacteria
Water
extract
No．
Raw
liquor
1∶1
dilution
1∶2
dilution
1∶4
dilution
1∶8
dilution
Negative
control
Positive
control
S． aureus D． fastuosa Ⅰ Lawn Lawn Lawn Lawn Lawn ○ Lawn
C． majus Ⅱ ○ ○ Lawn Lawn Lawn ○ Lawn
Shuang Huang Lian Ⅲ ○ ○ ○ Lawn Lawn ○ Lawn
E． coli D． fastuosa Ⅰ Lawn Lawn Lawn Lawn Lawn ○ Lawn
C． majus Ⅱ Colony Lawn Lawn Lawn Lawn ○ Lawn
Shuang Huang Lian Ⅲ ○ ○ Colony Lawn Lawn ○ Lawn
3 Conclusions and discussions
(1)The study results show that the poisonous plant Datu-
ra fastuosa L． has no inhibitory or killing effect on Escherichia
coli and Staphylococcus aureus． Related studies have shown
that Datura fastuosa L． has no contacting toxicity of insects，
and just has anesthetic effect． Thus it can be seen that it is in-
advisable to apply Datura fastuosa L． in agroforestry as a pesti-
cide plant，and it can only be used as a narcotic drug in the
medical application．
(2)However，Chelidonium majus L． has bactericidal ac-
tion． Compared with the control(Shuang Huang Lian) ，its bac-
tericidal effect is poor． Possible reasons are as follows:Cheli-
donium majus L． is poor self-sterilizing and is a kind of simple
recipe，but Shuang Huang Lian is a compound recipe． Polyp-
harmacy can make bactericidal action stronger and more com-
prehensive through reasonable compatibility of medicines．
(3)Shuang Huang Lian has stronger bactericidal action．
Another factor may also affect the concentration． Shuang
Huang Lian has passed the national certification with adult oral
10 ml for one time． Lower concerntratrion and inadequate do-
ses will not achieve the therapeutic effect． But Chelidonium ma-
jus L． liquid medicine needs easy preparation，which is simply
purified by using the Chinese medicine Yinpians cooking meth-
ods． Thus，it can be seen that preparation of the test medica-
ment raw liquor need further improvement in the future．
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(To page 32)
82 Medicinal Plant 2010
below:
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evant to the place where it grows．
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plant and the three-year-old plant although the amount of Fe in-
creased slightly during the third growth year． Significant differ-
ences were found in the content of Mn，Zn and Cu，when com-
paring the one-year-old plant to the two-year-old plant． Taking
those four elements and costs for consideration，the two-year-
old Dioscorea nipponica is the best for harvesting．
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不同产地及生长年限的穿龙薯蓣微量元素含量测定
王宏伟，李金玲，李攀登，王成章，郑友兰* (吉林农业大学中药材学院，吉林长春 130118)
摘要 ［目的］探讨穿龙薯蓣中微量元素含量与产地及生长年限的关系。［方法］采用火焰原子吸收光谱法对不同产地及生长年限穿龙薯蓣中
微量元素的含量进行测定。［结果］测定了 64个批次穿龙薯蓣中 Fe、Mn、Zn、Cu 4种主要微量元素的含量，结果表明其 Fe、Mn、Zn含量丰富，且 2
年及 3年生的穿龙薯蓣中微量元素含量相对较高，1年及 4年生含量相对较低。［结论］不同年生穿龙薯蓣中微量元素含量存在差异，且与产地
有一定相关性。综合考虑，在生产上采收 2年生穿龙薯蓣性价比最高。
关键词 火焰原子吸收光谱法;穿龙薯蓣;
欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁
微量元素
(From page 28)
白花曼陀罗·白屈菜水浸液杀菌效果研究
宋瑞佳，候伟倬，杨杰，冯静，张小娜 ，范红哲 (承德医学院，河北承德 067000)
摘要 ［目的］研究药用植物白花曼陀罗(Datura metel L．)和白屈菜(Chelidonium majus L．)对金黄色葡萄球菌和大肠杆菌水浸液的杀菌效果。
［方法］取当年生成熟白花曼陀罗和白屈菜植株，用沸水浸提法制成药液，以双黄连口服液为对照，对金黄色葡萄球菌和大肠杆菌进行杀菌研
究。［结果］白花曼陀罗对金黄色葡萄球菌和大肠杆菌均无明显抑杀作用;白屈菜对金黄色葡萄球菌和大肠杆菌有明显的抑杀作用，但对金黄
色葡萄球菌抑杀作用比对大肠杆菌的更强。［结论］白屈菜水浸液对金黄色葡萄球菌和大肠杆菌有明显的抑杀作用，而白花曼陀罗水浸液无杀
菌作用。
关键词 白花曼陀罗;白屈菜;水浸液;杀菌;大肠杆菌;金黄色葡萄球菌
23 Medicinal Plant 2010