全 文 ：Triterpenoids from the Dai Medicinal Plant Winchia calophylla
ZHU Wei-Ming1 ,2 , SHEN Yue_Mao1 , HONG Xin1 , ZUO Guo_Ying1 ,
YANG Xiao_Sheng1 , HAO Xiao_Jiang1＊
(1.Laboratory of Phytochemistry , Kunming Institute of Botany , The Chinese Academy of Sciences , Kunming 650204, China;
2.Department of Chemistry , Yunnan Normal University , Kunming 650092 , China)
Abstract:　A new compound(1)named as winchic acid and eight known ones were isolated from the stem
barks of Winchia calophylla A.DC.(collected in Xishuangbanna , Yunnan Province of China).The new
compound 1 was identified as 3β_hydroxy_27_(4_hydroxy_3_methoxy_E_cinnamoyloxy)_lup_20(29)_en_28_oic
acid on the basis of 1D and 2D NMR experiments.The eight known compounds were determined to be lu-
penone , lupenyl acetate , betulinic acid , α_amyrin acetate , ursolic acid , ptiloepoxide , β_amyrin and cycloeu-
calenol , respectively.
Key words:　triterpenoid;winchic acid;Winchia calophylla ;Apocynaceae
　　The traditional Dai medicinal plant Winchia calo-
phylla A.DC.(Apocynaceae), named as Mai_Ding_Jie(transliteration)or Ji_Jiao_Shu (free translation)in Dai
language , is distributed in Yunnan and Hainan provinces
of China , as well as in India , Burma and Indonesia[ 1] .
In Xishuangbanna , Yunnan Province of China , its leaves
and stem barks have been used in the treatment of chronic
tracheitis by the folks of Dai Nationality and contain effec-
tive agents as cough_ and wheeze_reliever and
expectorant
①.Previously , five indole_alkaloids were iso-
lated and characterized from W.calophylla [ 2 ,3] .Howev-
er , the relative bioactive components were not identified.
Thus we systematically investigated the chemical con-
stituents of this medicinal plant , especially the non_alka-
loid chemical components that were not reported before.
Here , we report nine triterpenoids obtained in the study.
1　Results and Discussion
The ethanolic extract of the stem barks of W.calo-
phylla were partitioned between petroleum ether and wa-
ter.The petroleum ether fraction was subjected to column
chromatography over Si gel H to afford nine triterpenoids ,
viz., 3β_hydroxy_27_(4_hydroxy_3_methoxy-E-cinnamoylo_
xy)_lup_20(29)_en_28_oic acid (1)(Fig.1), lupenone
(2)[ 4 ,5] , lupenyl acetate(3)[ 5 ,6] , betulinic acid(4)[ 7] ,
α_amyrin acetate (5)[ 8] , ursolic acid(6)[ 9] , ptiloepox-
ide(7)[ 10] , β_amyrin(8)[ 11] and cycloeucalenol(9)[ 12](Fig.2).Compound 1 was new , and named as winchic
acid.
Compound 1was obtained as crystals(CH3COCH3),
with mp 226 ℃ (dec.), [ α] 12D -14.7°(c 0.272 ,
CHCl3).The high_resolution EIMS exhibited the molecu-
lar ion peak at m/z 648.403 0 corresponding to the
molecular formula of C40H56O7 (calcd.648.402 6).The
1H_ and 13C_NMR data were similar to those of 3β , 27_di-
hydroxy_lup_20(29)_en_28_oic acid (10)(cylicodiscic
acid).The 13C_NMR signal atδ169.7 revealed the pres-
ence of a conjugated ester carbonyl group which was fur-
ther supported by the IR absorption at 1 702 cm-1.The
EIMS fragmentation at m/ z 471 ([M-177] +)and 177
([M-471] +)indicated that 1 was an ester formed from
cylicodiscic acid(10)with an acyl having the composition
of C10H9O3 (M+ , 177).The 13C_NMR signals at δ
55.9q , 111.5d , 115.6d , 116.9d , 124.0d , 126.5s ,
145.9d , 149.1s , 151.3s and 167.9s and the 1H_NMR
signals atδ3.73(3H , s), 7.38(1H , d , J =1.8 Hz),
6.86(1H , d , J =15.8 Hz), 7.19 (1H , d , J =8.2
Hz), 7.26(1H , dd , J =1.8 , 8.2 Hz)and 8.04(1H ,
d , J =15.8 Hz)revealed that the moiety of C10H9O3
should be E_3_(4_hydroxyl_3_methoxyl phenyl)_
propenoyl[ 13] .The 1H_13C long_range correlation between
the H_27(δ4.96 and 5.02)and carboxyl carbonyl C_1′(δ169.7)indicated that in 1 the E_3_(4_hydroxyl_3_
methoxyl phenyl)_propenoyl was linked to C_27 via an
oxygen (Fig.1).Moreover , compound 10 was obtained
after hydrolysis of 1 with KOH inMeOH_H2O[ 14 ,15] , sup-
porting the above structural elucidation.Therefore , com-
pound 1 was determined to be 3β_hydroxy_27_(4_hydroxy_
3_methoxy-E-cinnamoyloxy)_lup_20(29)_en_28_oic acid.
2　Experimental
2.1　General experimental procedures
Melting points were determined using an XRC_1 mi-
cro_melting point apparatus and uncorrected.Optical rota-
tions were determined on a JASCO_20 polarimeter.IR
spectra were obtained on KBr pellets using a Bio_Rad
FTS_135 spectrometer.The 1H_ and 13 C_NMR spectra
were obtained on BRUKER AM_400 and/or DRX_500.
1H and 13C chemical shifts were referenced to
chloroform_d1 atδH 7.24 andδC 77.0(using TMS as in-
ternal standard), respectively.One_bond heteronuclear
1H_13C connectivity was determined by proton_detection
HMQC experiments.Multiple_bond 1H_13C connectivity
Received:2001-04-05　Accepted:2001-06-14
Supported by the National Natural Science Foundation of China for Outstanding Young Scient ists to Hao Xiao_Jiang(39525025).
＊Author for correspondence.E_mail:.
① The Medicinal Office of Nationality of Prefecture of Xishuangbanna(西双版纳州民族医药调研办公室).The Medicinal Records of Dai Nationality in
Xishuangbanna.1980.168-169.(in Chinese)
植　物　学　报　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
Acta Botanica Sinica　2002 , 44(3):354-358
Fig.1.　The selected HMBC correlations for compound 1.
Fig.2.　The structures of compounds 2-10.
was determined by proton_detection HMBC experiments.
EIMS and HREIMS measurements were carried out on a
VG Auto Spec_3000 spectrometer.TLC and column chro-
matography were performed on plates precoated with Si gel
F254 and over Si gel H (Qingdao Marine Chemical Ltd.,
People s Republic of China), respectively.Solvents were
distilled prior to use.
2.2　Plant materials
The stem barks of Winchia calophylla A.DC.were
collected in Xishuangbanna , Yunnan Province of China in
July 2000.The plant was identified by Mr.CUI Jing_Yun
and the voucher specimen was deposited in the Xishuang-
banna Garden of Tropical Plants , the Chinese Academy of
Sciences.
2.3　Extraction and isolation
The dried and ground stem barks(10.5 kg)of W.
calophylla were extracted four times with 95%EtOH un-
der reflux.Removal of the solvents in vacuum produced a
concentrated syrupy which was partitioned between
petroleum ether and water.The petroleum ether fraction(76.8 g)was subjected to flash column chromatography
over Si gel H eluted with gradient petroleum ether_EtOAc
to afford six fractions.The fraction 1 (28.6 g)was fur
ther isolated by flash column chromatography over Si gel
ZHU Wei-Ming et al:Triterpenoids from the Dai Medicinal Plant Winchia calophylla 355　
Table 1　The 13C_NMR data for compounds 1-10
Carbon 　 11) 　 22) 　 32) 　 42) 　 52) 　 62) 　 72) 　 82) 　 92) 　 101)
1 39.4 39.7 38.5 38.4 38.6 39.2 38.6 38.7 30.8 39.6
2 24.8 34.1 23.8 26.5 23.6 27.9 27.2 27.3 34.9 24.4
3 77.9 218.0 81.0 78.2 80.9 78.1 78.3 79.0 76.7 78.4
4 39.6 47.4 38.4 38.4 38.4 39.2 38.7 38.8 44.7 39.6
5 56.2 55.1 55.5 55.1 55.4 55.7 55.2 55.3 43.4 56.3
6 18.9 19.8 18.2 17.9 18.3 18.6 18.1 19.3 24.7 19.0
7 36.1 33.7 34.3 34.0 32.9 33.5 34.0 32.8 25.2 36.2
8 42.1 40.9 40.9 40.3 40.1 39.9 40.8 38.8 46.8 42.2
9 52.5 49.9 50.4 50.2 47.7 47.9 50.2 47.7 23.6 52.5
10 37.9 37.0 37.2 36.7 36.9 37.2 36.9 37.2 29.6 37.7
11 21.5 21.6 21.0 20.5 23.4 23.5 21.2 23.6 27.0 21.7
12 26.0 25.3 25.2 25.2 124.4 125.5 26.0 121.8 33.0 25.9
13 39.5 38.3 37.8 38.0 139.7 139.5 37.8 145.2 45.4 39.6
14 46.2 43.0 42.9 42.0 42.2 43.4 42.0 41.7 49.0 47.0
15 28.4 27.5 27.5 30.2 28.4 28.6 26.3 26.2 35.4 28.4
16 33.5 35.6 35.6 31.8 26.7 24.8 33.4 27.0 28.1 34.2
17 56.5 43.0 43.0 55.8 33.8 47.9 36.0 32.5 52.3 56.8
18 50.1 48.4 48.0 46.6 60.6 53.5 42.0 47.3 17.8 50.4
19 47.8 48.0 48.4 48.9 39.6 39.4 35.9 46.9 27.2 48.0
20 151.2 150.8 150.9 150.2 39.6 39.2 151.3 31.1 36.2 151.6
21 31.2 29.9 29.9 29.2 31.3 30.9 55.7 34.8 18.4 31.4
22 37.4 40.0 40.0 36.6 41.6 37.3 63.7 37.2 35.1 37.9
23 28.6 26.7 28.0 28.2 28.1 28.6 27.8 28.1 31.4 28.7
24 16.5 21.0 16.5 15.6 16.7 15.5 15.3 15.5 156.9 16.4
25 17.0 16.0 16.2 15.5 15.7 16.3 16.0 15.6 33.9 17.0
26 17.0 15.9 16.0 15.3 16.9 17.3 15.7 16.9 22.0 17.2
27 63.4 14.6 14.5 14.1 23.3 23.8 14.5 26.0 21.9 60.3
28 179.1 18.1 18.0 178.6 28.7 179.4 14.8 28.4 19.2 179.5
29 110.2 109.4 109.3 108.8 17.5 17.3 112.0 33.3 14.4 109.9
30 19.7 19.4 19.5 18.6 21.4 21.2 27.0 23.7 106.0 19.7
CH3CO - - 21.2 - 21.2 - - - - -
CH3 CO - - 170.9 - 170.8 - - - - -
1)Spectra were obtained from 125MHz , and recorded in C5D5N at room temperature.The 13C_NMR data for the ester moiety of 1:167.9(1′), 115.6(2′),
145.9(3′), 126.5(4′), 111.5(5′), 149.1(6′), 151.3(7′), 116.9(8′), 124.0(9′), 55.9(OCH3).2)Spectra were obtained from 100 MHz , and
recorded in CDCl3 at room temperature.
H , eluting with petroleum ether_CH2Cl2 (5∶1)to yield
5 (10.5 g), 3(11.6 g)and 2(3.6 g).Similarly , frac-
tions 2(10.1 g)and 3(1.3 g)afforded 8(5.2 g)and 9(1.1 g), and 7 (20 mg)through flash column chro-
matography eluting with petroleum ether_CH2Cl2 (2∶1),
and the fraction 5(4.7 g)produced 4(1.8 g)and 6(52
mg)after eluting with petroleum ether_EtOAc(5∶1), and
the fraction 6(2.1 g)yielded 1(60 mg , petrol_EtOAc ,
2∶1), respectively.
2.4　Identification
Compound 1　C30H48O3 , crystal (CH3COCH3),
mp 226 ℃(dec.), [α] 12D -14.7°(c 0.272 , CHCl3).
IRνKBrmax cm-1:3 423 (OH), 1 702 (COOR), 1 629(C C), 1 597 , 1 514 (Ar ), 1 272 , 1 171(ArC H CHCOOR).HREIMS m/z 648.403 0(calcd.
for C30H48O3 648.402 6).EIMS m/ z(%):648 (M+ ,
7), 630(2), 587(3), 471(1), 454(13), 436(6),
408(3), 393(9), 234(9), 207(21), 194(69), 177
(100), 145(19), 119(11), 107(16), 81(20).1H_
NMR(500 MHz , C5D5N)δ:8.04 (1H , d , J =15.8
Hz , H_3′), 7.38(1H , d , J =1.8 Hz , H_5′), 7.26(1H , dd , J =1.8 , 8.2 Hz , H_9′), 7.19(1H , d , J=8.2 Hz , H_8′), 6.86(1H , d , J =15.8 Hz , H_2′),
5.02(1H , d , J =12.9 Hz)/4.96(1H , d , J =12.9
Hz)(H_27), 4.97 (1H , s)/4.77 (1H , s)(H_29),
3.73(3H , s , OCH3), 3.56 (1H , m , H_19), 3.42(1H , t , J =8.1 Hz , H_3), 2.95(1H , dt , J =2.8 ,
10.5 Hz , H_13), 2.71 (1H , d , J =12.7 Hz , H_
16β)/1.60(1H , m , H_16α), 2.26(1H , m , H_22α)/
1.57(1H , m , H_22β), 2.25 (1H , m , H_21β)/1.52(1H , m , H_21α), 2.13(1H , d , J =10.7Hz , H_2α)/
1.79(1H , d , J =10.7 Hz , H_2β), 2.05(1H , t , J =
10.2 Hz , H_18), 2.00 (1H , m , H_12β)/1.18(1H ,
m , H_12α), 1.85(2H , m , H_15), 1.81 (3H , s , H_
30), 1.71 (1H , m)/1.65 (1H , m)(H_7), 1.68(1H , m , H_1β)/1.00(1H , m , H_1α), 1.54(2H , m ,
H_6), 1.53(1H , d , J =11.8 Hz , H_9), 1.49(2H ,
m , H_11), 1.14 (3H , s , H_23), 1.12 (3H , s , H_
26), 0.99(3H , s , H_24), 0.95(1H , d , H_5), 0.86(3H , s , H_25).
Hydrolysis of 1　Compound 1 (20 mg)was re-
fluxed with 2% KOH in MeOH (20 mL)and H2O (2
mL)for 3 h.The concentrated mixture was acidified to
pH 1-2 with 2%HCl , and extracted with EtOAc.The
EtOAc solution was washed with water and dried over
Na2SO4.A white powder was yielded after the removal of
solvents.The powderwas subjected to column chromatog-
raphy over Si gel H eluting with gradient petroleum ether_
EtOAc to produce a crystalline powder which was identi-
fied to be cylicodiscic acid(10).EIMS m/z (%):472
356　 植物学报　Acta Botanica Sinica　Vol.44　No.3　2002
(M+ , 10), 454(21), 441(5), 423(15), 246(12),
234(15), 207(40), 189(34), 175(16), 135(38),
121(25), 107(29), 95 (38), 78 (96), 63(100).
1H_NMR(400MHz , C5D5N)δ:4.93(1H , br.s)/4.74(1H , br.s)(H_29), 4.60 (1H , d , J =12.3 Hz)/
4.21(1H , d , J =12.3 Hz)(H_27), 3.39 (1H , dd ,
J =8.2 , 8.0 Hz , H_3), 3.62(1H , m , H_19), 2.92(1H , m , H_13), 2.81(1H , d , J =12.5 Hz , H_1β),
2.46(1H , d , J =13.1 Hz , H_2α), 1.7 (3H , s , H_
30), 1.18 (3H , s , H_26), 1.14 (3H , s , H_23),
0.99(3H , s , H_24), 0.86(3H , s , H_25).
Compound 2　C30H48O , [α] 12D +61.1°(c 0.749 ,
CHCl3).EIMS m/ z(%):424(M+ , 76), 409 (27),
313(27), 245(23), 218(87), 205(79), 189(44),
175(26), 161(30), 149(40), 135(51), 121(64),
109(77), 95(80), 81(80), 69(82), 55(100).1H_
NMR(400 MHz , CDCl3)δ:4.67 (1H , d , J =2.2
Hz)/4.56(1H , d , J =1.2 Hz)(H_29), 1.67 (3H ,
s , H_30), 1.07 (3H , s , H_23), 1.06 (3H , s , H_
26), 1.04 (3H , s , H_24), 0.94 (3H , s , H_27),
0.92(3H , s , H_25), 0.79(3H , s , H_28), consistent
with those of lupenone
[ 4 ,5] .
Compound 3 　C32H52O2 , [ α] 12D +39.5°(c
0.247 , CHCl3).IRνKBrmax cm-1:1 735s(C O of Ac),
1 246s (C_O of AcO_).EIMS m/ z (%):468 (M+ ,
85), 453(20), 408(15), 393(12), 249(21), 218(83), 203 (37), 189 (100), 161 (25), 147 (29),
135(62), 121(66), 107(59), 95(69), 81(65), 69
(74).1H_NMR(500 MHz , CDCl3)δ:4.68(1H , br.
s)/4.57 (1H , br.s)(H_29), 4.47 (1H , dd , J =
6.2 , 8.5 Hz , H_3), 2.04(3H , s , Ac), 1.68 (3H ,
s , H_30), 1.03 (3H , s , H_26), 0.94 (3H , s , H_
27), 0.85 (3H , s , H_25), 0.84 (3H , s , H_23),
0.83(3H , s , H_24), 0.79(3H , s , H_28), consistent
with those of lupenyl acetate[ 5 ,6] .
Compound 4　C30H48O3 , [α] 15D +5.4°(c 0.278 ,
CHCl3).EIMS m/ z(%):456(M+ , 31), 438 (12),
410(8), 395 (5), 248 (33), 220 (21), 207 (32),
189 (40), 175 (55), 161 (52), 145 (44), 133(100), 123(71), 109 (79), 94 (60), 77 (73), 57
(76).1H_NMR(400 MHz , CD3OD+CDCl3)δ:4.77(1H , d , J =1.4 Hz)/4.71(1H , br.s)(H_29), 3.21(1H , dd , J =6.7 , 9.5 Hz , H_3), 3.06(1H , dt , J =
6.0 , 10.9Hz , H_13), 2.32(2H , br.d , J =12.8Hz ,
H_2), 1.99(2H , m , J =4.0 , 6.6 , 10.6Hz , H_16),
1.75 (3H , s , H_30), 1.04 (3H , s , H_26), 1.01(6H , s , H_23/H_24), 0.89 (3H , s , H_25), 0.81(3H , s , H_27), consistent with those of betulinic
acid[ 7] .
Compound 5 　C32H52O2 , [ α] 11D +71.1°(c
0.299 , CHCl3).IRνKBrmax cm-1:1 737s(C O of Ac),
1 245s (C_O of AcO_).EIMS m/ z (%):468 (M+ ,
16), 453(4), 218(100), 203(17), 189(15), 175(5), 147 (7), 135 (14), 121 (11), 109 (13), 93
(15), 81(14), 69(21).1H_NMR(400MHz , CDCl3)
δ:5.11 (1H , dd , J =3.5 , 3.5 Hz , H_12), 4.91(1H , dd , J =6.9 , 9.0 Hz , H_3), 2.04 (3H , s ,
Ac), 1.05 (3H , s , H_27), 1.00 (3H , s , H_26),
0.96(3H , d , J =4.5 Hz , H_30), 0.91(3H , d , J =
5.4 Hz , H_29), 0.86(3H , s , H_24), 0.85(3H , s ,
H_23), 0.79 (6H , s , H_25/H_28), consistent with
those ofα_amyrin acetate[ 8] .
Compound 6 　C30H48O3 , [ α] 15D +43.4°(c
0.173 , C5H5N).EIMS m/ z(%):456(M+ , 17), 438(7), 410(11), 392 (6), 300(5), 248 (100), 219(10), 207(56), 203(61), 189(29), 175(18), 147(19), 133(61), 119 (39), 105 (32), 93 (27), 81
(30), 69(38), 57(44).1H_NMR(400MHz , C5D5N)δ:5.49(1H , t , J =3.2 , 3.5Hz , H_12), 3.46(1H ,
dd , J =7.4 , 8.6 Hz , H_3), 2.63(1H , d , J =11.2
Hz), 1.24 (3H , s , H_27), 1.22 (3H , s , H_23),
1.04(3H , s , H_25), 1.02(3H , d , J =6.0 Hz , H_
30), 0.97(3H , s , H_24), 0.95(3H , d , J =6.0Hz ,
H_29), 0.88 (3H , s , H_26), consistent with those of
ursolic acid[ 9] .
Compound 7 　C30H48O2 , [ α] 12D +65.3°(c
0.185 , CHCl3).EIMS m/ z(%):440(M+ , 69), 422(25), 407(16), 357(49), 339(24), 271(13), 243(16), 231 (11), 217 (20), 207 (100), 189 (83),
175(28), 161(29), 149(29), 135(56), 121(38),
109(36), 95(41), 81(25), 69(21).1H_NMR(400
MHz , CDCl3)δ:5.06 (1H , s)/4.87 (1H , s)(H_
29), 3.46(1H , d , J =4.6 Hz , H_21), 3.08(1H ,
dd , J =7.6 , 8.0 Hz , H_3), 2.90(1H , d , J =4.7
Hz , H_22), 2.00(1H , q , J =7.0 Hz , H_19), 1.75(1H , m)/1.71(1H , m)(H_1), 1.77(1H , m)/1.29(1H , m)(H_15), 1.68(1H , m)/1.27(1H , m)(H_
16), 1.65(2H , d , J =3.2 Hz , H_2), 1.63(1H , dd ,
J =2.7 , 5.2 Hz , H_13), 1.55(2H , m , H_12), 1.54(1H , m)/1.40 (1H , d , J =3.7 Hz)(H_6), 1.53(1H , m)/1.32(1H , m)(H_11), 1.42(1H , br.s , H_
18), 1.38(2H , m , H_7), 1.31(1H , m , H_9), 1.05(3H , d , J =6.9 Hz , H_30), 1.02 (3H , s , H_26),
1.00(3H , s , H_23), 0.95 (3H , s , H_27), 0.85(3H , s , H_25), 0.81(3H , s , H_28), 0.80(3H , s ,
H_24), 0.70 (1H , d , J =9.1 Hz , H_5), consistent
with those of ptiloepoxide[ 10] .
Compound 8　C30H50O , [α] 11D +71.6°(c 0.349 ,
CHCl3).EIMS m/ z(%):426(M+ , 14), 411 (4),
300(2), 257(3), 218 (100), 203 (26), 189(12),
175(11), 161(7), 147 (10), 135 (14), 121(16),
107(17), 95(21), 81(18), 69(28).1H_NMR(400
MHz , CDCl3)δ:5.18(1H , dd , J =3.4 , 3.4 Hz , H_
12), 3.22(1H , dd , J =4.6 , 11.2 Hz , H_3), 1.13(3H , s , H_27), 0.97(3H , s , H_26), 0.95(3H , s ,
H_25), 0.94 (3H , s , H_23), 0.87(3H , s , H_24),
0.83 (6H , s , H_29/H_30), 0.79 (3H , s , H_28),
consistent with those of β_amyrin[ 11] .
Compound 9　C30H50O , [α] 11D +46.5°(c 0.409 ,
CHCl3).IRνKBrmax cm-1:1 641+886 ( C H2);EIMS
ZHU Wei-Ming et al:Triterpenoids from the Dai Medicinal Plant Winchia calophylla 357　
m/ z(%):426(M+ , 34), 408(78), 393 (52), 300(27), 283(12), 245(11), 201(14), 189(19), 175(27), 161(25), 147(32), 133(32), 121(38), 107
(48), 95 (65), 81 (59), 69 (98), 55 (100).1H_
NMR(400 MHz , CDCl3)δ:4.72 (1H , br.s)/4.66(1H , br.s)(H_30), 3.21 (1H , ddd , J =4.5 , 9.0 ,
10.5 Hz , H_3), 1.03(3H , d , J =6.8 Hz , H_27),
1.02(3H , d , J =6.8 Hz , H_26), 0.98(3H , d , J =
6.8 Hz , H_29), 0.97(3H , s , H_18), 0.90 (3H , d ,
J =6.3 Hz , H_21), 0.89(3H , s , H_28), 0.38(1H ,
d , J =4.1 Hz)/0.14(1H , d , J =4.1 Hz)(H_19),
consistent with those of cycloeucalenol[ 12] .
Acknowledgements:　All spectra were recorded by the
analytical group of the Laboratory of Phytochemistry ,
Kunming Institute of Botany , the Chinese Academy of
Sciences , China.
References:
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傣族药用植物盆架树中的三萜
朱伟明1 ,2　沈月毛1　洪　鑫1　左国营1　杨小生1 　郝小江1＊
(1.中国科学院昆明植物研究所植物化学开放研究实验室 , 昆明 650204;2.云南师范大学化学系 , 昆明 650092)
摘要:　从傣族药用植物盆架树(Winchia calophylla A.DC.)茎皮的石油醚可溶部分分离到 9个化合物 , 经现代波谱
技术证明 ,其中之一为新化合物 , 命名为盆架酸(winchic acid)。另 8 个已知化合物分别为羽扇豆烯酮 、乙酸羽扇豆
酯 、桦木酸 、乙酸-α-香树醇酯 、乌索酸 、ptiloepoxide、β_香树醇和 cycloeucalenol。
关键词:　三萜;盆架酸;盆架树;夹竹桃科
中图分类号:Q914　　　文献标识码:A　　　文章编号:0577-7496(2002)03-0354-05
收稿日期:2001-04-05　接收日期:2001-06-14
基金项目:国家杰出青年科学基金(39525025)。
＊通讯作者。E_mai l:.
(责任编辑:王　葳)
358　 植物学报　Acta Botanica Sinica　Vol.44　No.3　2002