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作 者 ：刘聪莉 张志芬 徐丽娟 孟祥霞 王晶珊

期 刊 ：植物学通报 1999年 16卷 04期 页码：420-424

关键词：甘薯；原生质体；植株再生；

摘 要 ：将甘薯(Ipomoea batatas (L．)Lam．)‘元气’和‘白星’(‘White Star’)的叶柄原生质体培养在含有0.05 mg·L-1 2,4-D和0.5 mg·L-1 KT的改良MS液体培养基中，3～4 d后细胞开始分裂。培养8～9周后，将直径达1～2 mm的愈伤组织转移到添加0.05～0.2 mg· L-1 2,4-D和0～0.5 mg·L-1 KT或添加0.5～2.0 mg ·L-1 NAA和1.0～3.0 mg·L-1 BAP 的MS固体增殖培养基上使其增殖。转移3～5周后，将愈伤组织再转移到MS基本培养基或转移到添加2.0～3.0 mg·L-1 BAP的MS培养基上。当进一步转移到MS基本培养基上后，从愈伤组织或从愈伤组织形成的不定根上再生出植株。‘元气’植株再生率高达60.0 ％，White Star高达43.4％。

Abstract:Protoplasts isolated from petioles of sweet potato ‘Cenki’ and ‘White Star’ were cultured in the modified liquid MS medium containing 0.05 mg· L- 1 2,4-D and 0.5 mg· L- 1 KT. The first cell division occurred within 3 to 4 days. After 8 to 9 weeks of culture, small calli derived from protoplasts were placed on the solid MS medium supplemented either with 0.05-0.2 mg· L-1 2,4-D and 0-0.5 mg·L- 1 KT or with 0.5-2.0 mg·L -I NAA and 1.0-3.0 mg·L- 1 BAP for callus proliferation. Furthermore, the calli were transferred on to either the hormone-free medium or the medium supplemented with 2.0-3.0 mg· L- l BAP, followed by being transferred on to the hormone-free medium for plant regeneration. The frequency of protoplast-derived calli regenerating plants reached 60.0% in ‘Genki’ and 43.3% in ‘White Star.

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