Abstract:Fluorescence in situ Hybridization(FISH) is a kind of technology
of non_radioactive in situ Hybridization, which have developed
in 80‘S . As a new molecular cytological method, it has widely been used in cellular genetics and molecular biology. This article briefly summarized basic principle and trait of FISH and the application of FISH in botany, including identification of alien chromatin, physical mapping of genes, chromosome RNA and genome evolution.