The Na+/H+ antiport genes named TaNHX1 and TaNHX2 were cloned by screening a salt stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed that TaNHX1 was 2 029 bp in length and contained a complete ORF of 1 638 bp. The TaNHX1 encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI. TaNHX2 was 1 693 bp in length consisting of a partial ORF followed by a 3′ UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. ART PCR assay showed that the level of TaNHX1 transcripts was increased and reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.
小麦Na+/h+反转运蛋白基因的克隆和特性
王子宁1,2 张劲松1 郭北海2 何锶洁1 田爱国1 陈受宜1*
(1. 中国科学院遗传研究所植物生物技术重点实验室,北京100101;2. 河北农林科学院粮油作物研究所作物育种室,石家庄050031)
关键词: 小麦;Na+/H+ 反转运蛋白基因;盐诱导
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