RAPD assessment of genetic variations in 33 varieties of banana ( Musa nana Lour.) was carried out. Eighteen primers were screened from 249 10 bp arbitrary primers, and a total of 192 DNA bands were amplified, among which 183 (95.31%) were polymorphic. The average number of DNA bands amplified by each primer was 10.67, and the average genetic distance among 33 varietieswas 0.3412. Based on UPGMA cluster analysis of 192 DNA bands amplified by 18 primers a DNA molecular dendrogram was established for 33 varietiesof banana in China, which divided the 33 varieties into four groups: group A,B,C and D. Group A included 20 varieties, group B included 5 varieties, group C included 2 varieties and group D included 6 varieties. Group A could be divided into 3 sub-groups: A1, A2 and A3. The molecular foundation of genetic diversity of banana was also explored.
香蕉33个品种的RAPD研究
杜道林1,2 苏杰1 周鹏2 郑学勤2 黄秉智3 李丰年3
（1. 海南师范学院生物系，海口571185；2. 中国热带农业科学院热带作物生物技术国家重点实验室，海口571101；3.广东省农业科学院果树研究所，广州%!510640）
摘要：利用RAPD技术对香蕉(Musa nana Lour.) 33个品种的遗传变异进行了研究.从249个随机引物中筛选出18个有效引物,用它们共扩增出192条DNA带,其中183条为多态性带,占95.31%,平均每个引物扩增的DNA带数为10.67条.利用18个有效引物扩增的192条DNA带对香蕉33个品种间的亲缘关系进行UPGMA聚类分析,计算出33个品种间的平均遗传距离为0.341*!2.在此基础上建立了香蕉33个品种的DNA 分子系统树状图.该系统将香蕉33个品种划归A、B、C和D 4个群,其中A群20个品种,B群5个品种,C群2个品种,D群6个品种;A群又可以分为3个亚群.对香蕉遗传多样性分子基础进行了探讨.
关键词： 香蕉；品种；RAPD；遗传多样性
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