Abstract:Plasmid pJIT101 containing bar gene and luc gene was transferred into wheat protoplasts mediated with PEG 1450. Transformants were then selected out in medium containing 50 mg/L Basta, a phosphinothricin (PPT)containing chemical product. The results of DNA hybridizaion indicated that both bar gene and luc gene had integerated into transformant genome. The luciferase activity has also been detected in those transformants.