Abstract:pBinLK carried two insecticidal genes, pea lectin (P-Lec)gene and soybean Kunitz trypsin inhibitor (SKTI) gene, were successfully transferred into 4 upland cotton ( Gossypium hirsutum L. ) cultivars, "Xinluzao-1 ", "Xinluzhong-2", "Jihe-321" and "Liao-9" via Agrobacterium-mediated transformation. Hypocotyl segments from aseptic seedlings were used as receipient. After co-cultivation of hypocotyl segments with A. tumefac/ens (Smith et Townsend) Conn, kanamycin-resistant calli were screened, and somatic embryos and regenerated plants were obtained through various media. Transgenic cotton plants harboring two insecticidal genes were confirmed by NPT-Ⅱ ELISA, PCR and PCR Southern. rllae results of bioassay demonstrated that the transgenic plants showed significant resistance to the larvae of cotton bollwonn (Heliothis armigera Hubner).