Abstract:The electrophoretograms of ghadin and high molecular weight (HMW) glutenin in the five octoploid amphiploids of wheat-wheatgrass were analyzed. The results indicated that the octoploid amphiploids of wheat ( Triticum aestivum L. )-wheatgrass( Agropyron intermedium (Host) P.B. = Elytrigia intermedia (Host) Nevski = Thinopyrum intermedium (Host) Barkwarth and Dewey) Zhong 1 and Zhong 2 had the same band patterns, and then the octoploid amphiploids of wheat-wheatgrass Zhong 3, Zbong 4 and Zhong 5 had the same band patterns which were different from Zhong 1 and Zhong 2. One band pattern of HMW glutenin which lack in wheat parents were occurred in Zbong 1, Zhong 2 and wheatgrass. New band patterns which were different from both of wheat and wheatgrass may also occur in Zhong 1 and Zhong 2. The authors found that the genome X of wheatgrass expressed one specific band pattern occurred in Zhong 3, Zhong 4 and Zhong 5. Authors analyzed the genomic constitution and wheat parents of the five octoploid amphiploids of wheat-wheatgrass by their band patterns of gliadin and HMW glutenin. Furthermore, the potential significance of the octoploid amphiploids of wheat-wheatgrass for wheat breeding was discussed.