Abstract:Protoplasts were enzymatically isolated from the cultured cell line No. 246 of Panax ginseng and suspended in a 67V-D liquid medium. After 35 days of culture, the cell clusters derived from protoplasts were transfered to a 67V-E semisolid medium. When the cell clusters grew to 2 mm approximately, they were transfered to common 67V medium for the callus regeneration.