Abstract:A salt-tolerant cell line of wheat ( Triticum aestivum L. ) that could grow well under 1.5% NaC1 stress was obtained by gradual increase of NaC1 concentration. Under NaC1 stress, the water content in the salt-tolerant cell line decreased less than that in the salt sensitive cell line served as control, while the I-I202 content and O2- production rate in the control cell line increased more obviously than those in the salt-tolerant cell line. Meanwhile, the membrane relative permeability, membrane lipid peroxidation and deesterification increased more significantly than those in the salt-tolerant cell line. These results indicated that the damage of wheat cell lines caused by NaCI stress had close relationship with active oxygen-mediated membrane lipid peroxidation and deesterification, and the resistance to the active oxygen mediated damage in the salt-tolerant cell line was remarkably higher than that in the control cell line.