Abstract:Genetic diversity, genetic differentiation and gene flow among 6 Cyclobalanopsis glauca (Thunb.) Oerst. populations in East China were investigated using the assay of vertical slab polyacrylamide gel electrophoresis. C. glauca maintained relatively high level of genetic variation as compared with the average plant species. Expected heterozygosity at species and population level was 0.225 2 and 0.221 6 respectively, and observed heterozygosity were 0. 166 1 and 0. 177 1 respectively. Genetic differentiation (GsT) was low among populations. GsT was 5.6%, and the means of genetic identity and genetic distance were 0.972 9 and 0.027 6 respectively. The divergence times among populations were from 14 000 to 270 000 years. The gene flow were 4.21 and 20.49 respectively.