Abstract:Two chimeric genes--CAT and GUS genes under the control of CaMV 35S promoter were transferred into the protoplasts isolated from cultivars of rice, corn, tobacco, carrot and carnation grown in China by electroporation and PEG pathway. Transient expression of these genes were obtained in all transformants. With electroporation, most of transformed corn, tobacco and carrot protoplasts showed CAT or GUS activity and the effeciency was related to parameters of the electroporater. In PEG pathway, the time of PEG incubation was shorter than those reported in most papers and positive results were obtained.