Abstract:The gene fragments of wheat (Triticum aestivum L. cv. Yang Mai No.4) high-molecularweight (HMW) glutenin subunit were amplified in vitro using two chemically synthetic oligonucleotide primers and Taq DNA polymerase after 35 cycles of polyrnerase chain reaction. The amplified fragments were cloned into plasmid Bluescript SK+ and the sequence of 608 bp inserted fragment was obtained in two directions. The sequence information has shown that the 608 bp fragment included downstream region of CAAT box and coding region for 166 N-terminal AA of wheat HMW glutenin subunit. As compared with HMW glutenin subunit genes from other species of wheat, it shows that the fragment amplified is a new HMW glutenin gene fragment.