Abstract:White and soft calli were induced from the stemnodes of Angelica dahurica on MS medium containing lmg/L 2,4-D, and subcultured on the same medium with decreased concentration of the hormone for about half a year, until quite a number of embryogenic cell clusters were produced in calli. Protoplasts prepared only from this kind of callus were regenerable. The protoplasts-derived colonies were able to develop into embryos directly or to grow continously into calli as affected by the hormone and, in particular, by osmotic pressure in the culture medium. The embryos either formed directly or via callus stage were all capable of regenerating complete plants under proper culture conditions.