Abstract:This paper describes a method for observing and identifying the cell wall protein- hydroxyproline-rich glycoprotein (HRGP) which has been characterized as an extensin. The HRGP prepared from carrot callus was sprayed on a mica sheet by using a simplyzed spraying device, After rotatory shadowing, HRGP was observed under electron microscope and it appeared as a rod-like molecule, Measurement showed that the molecular length of HRGP was about 87 nm.