Abstract:Permeability of various metabolites in photorespiratory carbon metabolism pathway is ex-amined by O2 electrode and spectrophotometry. The results show that O2, glycolate, glyoxyla-te, serine, asparate and α-ketoglutarate are permeable to the peroxisomal membrane for matrixenzymes activity, and the peroxisomal membrane seems to be an entry barrier to glutamate andNADH. The integrity of peroxisoma membrane is disrupted rapidly by Percoll existing pero-xisome suspension. The activity of hydroxypyruvate reductase and malate dehydrogenase is alsodecreased by Percoll.