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A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources


The glutamate oxaloacetate transaminase isozymes (GOT) extracted
from 125 biotypes of Citrus and its relatives, Fortunella, Poncirus and Microcitrus
were analyzed using polyacrylamide gel electrophoresis to investigate the taxonomic
relationships among citrus plants. Besides all the isozymes reported before, two new
bands were detected and designated  their putative alleles B and C in GOT-l.
Among pummelo cultivars wide variations were found. Most of the mandarins
were identical,  having  SS for GOI-1  and MM  for GOT-2  except  for
Zou-Pi-Gan and Yao-Gan, which both had FS at GOT-1 and might be hy-
brids. This finding suggests that all of the mandarins may have originated from a
common ancestor. Sour orange biotypes showed a considerable variation in GOT
isozymes. Most of the sour oranges in China were assumed to be hybrids between
pummelo and mandarin based on GOT isozyme patterns,  but two biotypes,
Xiao-Hong-Cheng and Zhu-lan, had FS at GOT-1 and MB at GOT-2, which
strongly suggests that they be hybrids of pummelo and Ichang papeda because B
allele of GOT-2 occurs only in Ichang papeda and its close relatives Yuzu and
Ichang lemon. From this study Yuzu is assumed to be derived from hybridization
of Ichan, papeda and mandarin.


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