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Optimization of fermentation conditions for polysaccharide of Tricholoma matsutake using response surface methodology

响应面法优化松茸发酵产多糖的培养条件



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ChineseJournalofBioprocessEngineering
Vol.9No.4
Jul.2011
doi:10.3969/j.issn.1672-3678.2011.04.001
OPQI
:2011-04-03
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Optimizationoffermentationconditionsforpolysaccharideof
Tricholomamatsutakeusingresponsesurfacemethodology
HAOJiwei,QIUFengtong
(ColegeofLifeScience,LinyiUniversity,Linyi276005,China)
Abstract:Responsesurfacemethodologywasadoptedtooptimizefermentationconditionsforpolysac
charideofTricholomamatsutake.Firstly,basedontheresultsofcarbonsourcesandnitrogensource
experiments,aPlacketBurmandesignwasusedtoinvestigatetheefectsofdiferentfactorsonpro
ductionofpolysaccharide,andthreesignificantfactorswerecornflour,soybeanmeal,and
KH2PO4.Thepathofsteepestascentwasusedtodefineoptimalresponseregionforthesethreefac
tors.OptimallevelcombinationsoffactorswereobtainedthroughBoxBehnkendesign.Theoptimal
fermentationconditionsforpolysaccharideofTricholomamatsutakewereasfolows:cornflour
454%,soybeanmeal496%,KH2PO4015%,MgSO4·7H2O005%,VB10001%,initial
pH5.5,rotationspeed180r/min,andfermentationtime10d.Underoptimalfermentationcondi
tions,theyieldofpolysaccharidereached597g/L.
Keywords:experimentaldesign;fermentation;polysaccharides;Tricholomamatsutake
  
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w(
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w(MgSO4·7H2O)/


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pH




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ü
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ρ(45)/
(g·L-1)
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2 1 -1(500) 1(010) 1 -1 -1 -1(55) 1 1 1(15) 446
3 -1(200) 1 1 -1 1(010) -1 -1 -1 1 1 307
4 1 1 -1 1 -1 1(003) -1 -1 -1(180) 1 378
5 1 -1 1 1 1 -1 1 -1 -1 -1 542
6 1 1 1 -1 1 1 -1 1 -1 -1 461
7 -1 1 1 1 -1 1 1 -1 1 -1 362
8 -1 1 -1 1 1 -1 1 1 -1 1 332
9 -1 -1 -1 1 1 1 -1 1 1 -1 353
10 1 -1 -1 -1 1 1 1 -1 1 1 471
11 -1 -1 1 -1 -1 1 1 1 -1 1 395
12 -1 -1 -1 -1 -1 -1 -1 -1 -1 -1 321
  
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B -04817 -1521 0042
C 04317 1363 0047
D 00983 311 0198
E 02750 868 0073
F 01217 384 0162
G 03917 1237 0051
H 00083 026 0836
J -01517 -479 0131
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steepestascentpath
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w(
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)/

w(
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)/

w(KH2PO4)/

ρ(45)/
(g·L-1)
1 200 700 005 426
2 300 600 010 498
3 400 500 015 527
4 500 400 020 502
5 600 300 025 453
2.4 
WXY‹Œ|}‚c3[“”
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correspondingresults
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X1
w(
ã¬â

X2
w(
˜ó

X3
w(KH2PO4)
ρ(45)/
(g·L-1)
1 -1(300)-1(400) 0(015) 425
2 -1 1(600) 0 472
3 1(500) -1 0 566
4 1 1 0 537
5 0(400) -1 -1(010) 491
6 0 -1 1(020) 519
7 0 1 -1 549
8 0 1 1 503
9 -1 0(500) -1 470
10 1 0 -1 547
11 -1 0 1 438
12 1 0 1 546
13 0 0 0 569
14 0 0 0 578
15 0 0 0 582
E!Í,-
(P<0001),
N@ÊOQ,-
(P>005),
X1、X2、X3、X1X2、X1X3、X2X3、X

1、X

2、X

3AÊöX1X3–
gPÊQ,-RZ,-

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Table5 ANOVAanalysisforregressionequation
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H ~ç

H6*Ð
pE
  35696 9 03966 813024 <00001
X1 19110 1 19110 3917347 <00001
X2 00450 1 00450 92245 00288
X3 00325 1 00325 66647 00493
X1X2 01444 1 01444 296003 00028
X1X3 00240 1 00240 49248 00772
X2X3 01369 1 01369 280629 00032
X21 07742 1 07742 1587082 <00001
X22 03444 1 03444 706012 00004
X23 03388 1 03388 694501 00004
\P
00244 5 00049
N@Ê
00155 3 00052 11673 04922
hþÿ
00089 2 00044
é¤Z
35940 14
 
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ùZ,-
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-00365,X3=-00255,p­e*ã¬â、˜ó
KH2PO463VA6y~p©]Hù454%、496%

015%,
ñWez)*H6f]Hù
589g/L。
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MgSO4·7H2Oy~p©005%、
VB1y~p© 0001%、”• pH55、Wúª¬
180r/min
ñf12ûü
10d
ºTxgwx

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9:

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¥¦§¨‡nN©ª«¨‡nN
†]^ab`‡cˆW
Fig.3 Efectsofcornflourandsoybeanmealon
productionofPTM
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Fig.4 EfectsofcornflourandKH2PO4on
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