作 者 ：王昌全,郭燕梅,李冰,袁大刚,张济龙,林正雨,唐敦义
期 刊 ：生态学报 2008年 28卷 11期 页码：5377~5384
Keywords:Cd stress, hybrid rice, parents, MDA,
摘 要 ：以15份杂交水稻及其亲本为材料，采用水培试验研究了不同Cd2+浓度胁迫对水稻叶片丙二醛（MDA）含量的影响。结果表明：（1）在不同Cd处理水平和生育时期，保持系叶片MDA含量存在差异，如D83B叶片MDA含量各处理显著高于对照（p<0.05），MDA积累水平较高，受到Cd严重胁迫，而Ⅱ-32B在两个生育时期受低Cd浓度的影响不大，与对照差异不显著，且随着Cd浓度的增大，MDA含量均表现出较低的MDA积累水平，因此保持系Ⅱ-32B是一种较好的保持系材料。（2）Cd胁迫对恢复系各材料叶片MDA的影响不同，其中以R527、R498叶片MDA积累最明显，孕穗期和灌浆期各处理均显著高于对照，其余恢复系材料受不同Cd2+浓度的影响，MDA积累不稳定。（3）同一保持系（Ⅱ-32B）与不同恢复系（R498、R549、R892）组合配制的3个杂交稻叶片在MDA含量的增加幅度上表现不同，其中Ⅱ优498、Ⅱ优892受Cd影响较小，无论在孕穗期还是灌浆期叶片MDA含量增幅相对较小，而Ⅱ优549受Cd严重胁迫，各处理均显著高于对照，MDA 积累增幅最大。（4）同一恢复系（R498）与不同保持系（Ⅱ-32B、D62B）配制的杂交稻叶片MDA含量亦不同， 如D优498仅在孕穗期低浓度下MDA较对照有所降低，其余各处理MDA均积累较多，而Ⅱ优498MDA含量随着Cd浓度的增加，MDA增幅相对较小，可见水稻亲本对子代MDA的积累存在差异，可根据优良亲本，寻找优势组合，培育出抗Cd性强的杂交水稻。
Abstract:The content of malondialdehyde(MDA) in leaves of fifteen hybrid rice and their parents was here measured to obtain an understanding of the effects of Cd stress on the growth of rice employing the method of solution culture.(1)MDA content in leaves of the rice varied with the maintainer,Cd stress level and growth period.For example,D83B maintainers had higher MDA content in leaves by comparing with the control and were stressed severely,while the content of MDA in leaves of Ⅱ-32B maintainers varied slightly in comparison with the controls,and did not increase significantly with the increase of Cd content in the solution.The results implied that Ⅱ-32B was a good maintainer.(2)The effects of Cd stress on the rice depended on the restorer lines.The content of MDA in leaves of restorer lines R527 and R498 was significantly higher than that of the control at the booting and filling stages.While other restorers were influenced by Cd stress and varied with the restorer lines and the growth stage.(3)The contents of MDA in leaves of the hybrid rices from Ⅱ-32B and different restorer lines(R498,R549 and R892) showed the different increasing amplitude.Ⅱ You 498 and Ⅱ You 892 were not significantly influenced by Cd stress,while the Ⅱ You 549 was significantly influenced by Cd stress,of which the MDA content was significantly higher than that of the controls,and accumulated higher content of MDA in leaves.(4)The contents of MDA in the hybrid rices depended on the cross combination from the restorer R498 and the maintainers Ⅱ-32B and D62B.D You 498 had lower MDA content only at the booting stage and the lower Cd content by comparing with the control, while the contents of MDA were significantly higher than that of the control at other stages and Cd contents.Meanwhile,the content of MDA in leaves of Ⅱ You 498 increased with the Cd content in the solution.it was suggested that the content of MDA in leaves of the offspring be influenced by not only Cd stress,but also the parents,and the variety of anti-Cd hybrid rice be cultivated through the superiority combination and high-quality parents.
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