---

作 者 ：李金寒, 商博, 梁丹丹, 鲁志松, 刘阳

期 刊 ：核农学报 2016年 30卷 7期 页码：1316-1322

关键词：柠檬醛；黄曲霉毒素；ROS；电化学传感器；抑制机理；

Keywords:citral, aflatoxin, ROS, electrochemical sensor, inhibition,

摘 要 ：为探究ROS在柠檬醛抑制黄曲霉产毒过程中的作用,对柠檬醛作用下黄曲霉细胞释放ROS的含量、胞内ROS水平、抗氧化酶类活性和细胞氧化损伤程度的变化进行监测。结果表明,柠檬醛会使黄曲霉细胞内ROS含量明显增多,并部分释放到胞外。与甘油处理组相比,柠檬醛处理组的黄曲霉毒素含量明显较低,柠檬醛作用下的黄曲霉胞内抗氧化酶(CAT、SOD和GSH-Px)活性明显增强,胞内MDA和蛋白质羰基含量明显增大。扫描电镜观察发现,柠檬醛处理组的细胞损伤程度明显高于甘油处理组。综上,黄曲霉在柠檬醛作用下产生ROS,造成黄曲霉氧化损伤,从而抑制黄曲霉毒素的产生。本研究结果对粮食仓储具有一定理论指导意义。

Abstract:In order to understand the role of ROS in the citral-induced inhibition of aflatoxin production, ROS released from Aspergillus flavus, intracellular ROS level, activities of anti-oxidative enzymes (CAT, SOD and GSH-Px) and oxidative damage of protein and lipids were studied. It is observed that with citral treatment the concentration of intracellular ROS and the activity of anti-oxidative enzymes increase as well as the MDA and protein carbonyl contents. The results also showed that the concentration of aflatoxin declined and the cell membrane of Aspergillus flavus was damaged significantly after stimulated by citral. Based on the results, the mechanism for citral-induced inhibition of aflatoxin production in Aspergillus flavus, which involves the generation of ROS, formation of intracellular oxidative stress and oxidative damages of biomolecules, was proposed. And this study made fundamental knowledge for using citral in cereals storage.

---