摘 要 ：为探索羊栖菜中分离的各组份多糖体外抗氧化活性,将DEAE-52分离纯化得到的羊栖菜多糖活性组份进行红外光谱扫描确认,并评价DPPH·、·OH、O2-自由基的清除能力,还原力和抗脂质过氧化力。结果表明,去蛋白多糖经DEAE-52分离纯化、0~2 M NaCl溶液梯度洗脱得到3个主要多糖组份,其中红光谱扫描发现SFPSⅠ(0.5 M NaCl)、SFPSⅡ(0.75 M NaCl)、SFPSⅢ(1.0 M NaCl)均具有糖类特征吸收峰。3个组份均表现出较强的抗氧化活性,其中SFPS Ⅲ抗氧化活性最高,DPPH·的IC50 为0.499 μg,·OH 的IC50 为0.48 μg,O2-的IC50 为112.13 μg;还原力最强;抗脂质过氧化能力显著优于对照品BHT。研究结果为羊栖菜活性多糖作为天然抗氧化剂及功能性食品开发提供了理论基础。
Abstract:The study aims to explore the antioxidant capacity of polysaccharide fraction from Sargassum fusiforme in vitro. Polysaccharide fractions with biological activity from Sargassum fusiforme were separated by DEAE-52, and the components polysaccharide were scanned by infrared spectral. The purified polysaccharides were evaluated on their antioxidant activities including DPPH assay,superoxide radical assay,hydroxyl assay,lipid peroxidation and reducing capacity assay. Gradient elution of 0 to 2M NaCl chromatography applied to non-protein polysaccharides led to 3 major constituents, of which compounds SFPSⅠ(eluted by 0.5M NaCl)、SFPSⅡ(eluted by 0.75M NaCl)、SFPSⅢ(eluted by 1.0M NaCl) showed sugar characteristic absorption peaks in infrared spectrum and strong anti-oxidation activity. SFPS Ⅲ had the best anti-oxidation activity with DPPH·IC50 0.499μg, ·OH IC50 0.48μg, O2- IC50 112.13μg respectively, strong reducibility, and its ability to resist lipid peroxidation outperformed reference substance BHT. The study laid theoretical foundation for the development of Sargassun Fusiforme Polysaccharides in natural antioxidants and functional foods.