Abstract:In order to develop the application of endophytic biological control of Alopecurus japonicas Steud., the antifungal activity and taxonomic status of strain KMN-1 and KMN-11 was studied. KMN-1 and KMN-11 were significant antifungal activity against Fusarium head blight in vitro antagonism test. By the assay of fermentation from KMN-1 strain and KMN-11 strain exhibited fungi activity, the value of EC50 inhibiting mycelia growth against Fusarium graminearum was 6 572.97 mg·L-1、5 218.34 mg·L-1, respectively. The antibiotic bioactivity of metabolites from strain KMN-1 and KMN-11 was studied in vitro and vivo bioassay. The fermentation filtrate of strain KMN-1 had exhibited effect on controlling Fusarium graminearum, the efficacy of protective and curative efficacy were above 78.13% and 76.52% in wheat spikelet test respectively; In field experiment, spraying the fermentation filtrate of strain KMN-1 and KMN-11 can also reduce the epidemic degree. By morphological characteristics and molecular identification, KMN-1 strain is identified as Alternaria tenuissima and KMN-11 strain is identified as Fusarium incarnatum. Studies show that the prospects for biocontrol strains which separated from Fusarium spp. and Alternaria are very broad, and also laid the foundation for the further research and development potential of agricultural antibiotics.