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作 者 ：张明永， 段俊， 梁承邺

期 刊 ：热带亚热带植物学报 1999年 7卷 4期 页码：318~322

关键词：呼吸作用；自由基；雄性不育；水稻；

Keywords:Respiration, Reactive radicals, Cytoplasmic male sterility, Rice,

摘 要 ：用呼吸电子传递细胞色素途径的抑制剂氰化钾（KCN）与抗氰呼吸途径的抑制剂水杨基氧肟酸（SHAM）处理水稻细胞质雄性不育系（CMS）珍汕97A及其保持系珍汕97B的幼穗和花药后，KCN使不育系与保持系的超氧阴离子自由基（O₂^-.）产生受到抑制，不育系的O₂^-.的形成受抑制较多。SHAM处理则增高O₂^-.以不育系的增加较多。KCN与SHAM处理后都使不育系与保持系的丙二醛（MDA）含量升高，KCN使保持系的MDA含量升高较多，SHAM则使不育系的MDA含量升高较多。KCN处理后，不育系与保持系的超氧物歧化酶（SOD）活性下降，SHAM理后不育系与保持系的SOD活性变化不明显。H₂O₂处理对不育系与保持系幼穗的呼吸速率影响不大．H₂O₂＋FeSO₄处理后，使呼吸速率大幅度下降，表明H₂O₂＋FeSO₄所形成的羟自由基（·OH）比H₂O₂对呼吸代谢的破坏作用更大。

Abstract:The superoxide radical (O₂^-.) formation rates in the young panicles and anthers ofcytoplasmic male sterile (CMS) rice Zhenshan 97A (97A) and its maintainer Zhenshan 97B(97B) were inhibited by KCN, and accelerated by salicylhydroxamic acid (SHAM), but thepercentage of O₂^-. formation inhibited by KCN or accelerated by SHAM in sterile rice 97Awas more than that in fertile rice 97B. The malondialdehyde (MDA) content in both of 97A and 97B increased after KCN or SHAM treatment, but the great change of MDA was found in 97B treated by KCN or in 97A treated by SHAM. Superoxide dismutase (SOD) activity was inhibited by KCN, but was not affected by SHAM. The respiratory rates of 97A or 97B decreased after H₂O₂＋FeSO₄ treatment H₂O₂ while it did not change by treatment alone.

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