Abstract:The main research progresses on Phalaenopsis in vitro culture were reviewed, including explants selection, embryogenesis and protocorm-like body induction, medium, culture condition and method, and browning control, eg. Meanwhile, the bioreactor, genetic transformation, mutation breeding and other biotechnology were summarized. The flower stalk, tip of shoots and seeds from adult plants were common used as explants, and do the leaf, tip of shoots, root tips from tube plantlets. The main factors of explants browning were type of medium, pH, temperature and the physiological state. The researches about mutational breeding and genetic transformation in Phalaenopsis were still at preliminary stage.