Abstract:The embryos, endosperm and young leaves of 3-month-seedling of Ginkgo biloba L. were used as explants for callus induction experiment. Calli induced from embryos on MS medium supplemented with 3 mg L-1 NAA and 5 mg L-1 KT gave highest growth rate (100%), whereas calli induced from endosperms on MS with 3 mg L-1 NAA and 2 mg L-1 KT gave 42.3% growth rate. MS with 1 mg L-1 NAA and 3 mg L-1 BA was best for callus induction from leaves. The content of flavonoid compounds was highest in calli from embryos, and lowest in calli from embryos, and lowest in calli from endosperms.