Abstract:The acute toxicities of P. globosa on Artemia sinica, Brachionus plicatilis, Moina mongolia, Epinephelus akaara and Penaeus vannamei were studied using the method of semi-static water, and the poisoning route were discussed. P. globosa had different toxicity on the five aquatic animals. The survival of B. plicatilis decreased significantly (P<0.05) in algal culture, re-suspension and hemolytic toxins extraction of P. globosa for 72 h, whereas only motility of A. sinica was slightly inhibited by algal culture and re-suspension. Many algal cells in the alimentary canal of A. sinica and B. plicatilis, it suggested P. globosa cell could be fed by them. The algal culture, cell-free medium and cell contents had remarkable effects on the survival of larval E. akaara, but not cell fragments and hemolytic toxins extract of P. globosa, which implied that harmful substances were not attached to the algal cell surface, but inside the algal cell, and that P. globosa could excrete other harmful substances than hemolytic toxins into water to imperil surrounding organisms. After exposured in algal culture, re-suspended cells, filtrate and hemolytic toxins from P. globosa for 72 h, respectively, motilities of M. mongolia were all slightly different from the control though some M. mongolia died during the course. The P. vannamei, exposured in components from P. globosa algal culture for 72 h, were similar to M. mongolia. These suggested that P. globosa had few negative impacts on the survival of M. mongolia and P. vannamei. In conclusion, P. globosa had different effects on various aquatic animals by being ingested or excreting harmful materials into the water.