Abstract:The cucumber (Cucumis sativus L.) inhibiting axillary bud growth gene, CsCCD7, had been cloned (GenBank accession number: HQ005419) using by RACE, and its bioinformatics and semi-quantitative RT-PCR analysis were studied. The results showed that the CsCCD7 was the homologue gene of AtMAX3/CCD7 in Arabidopsis thaliana, which contain ORF with 1665 bp, and code the protein with 554 amino acids, named CsCCD7, belonging to CCD protein family. The secondary and tertiary structure prediction of CsCCD7 showed having rich β sheets, β turns and irregular curls, which indicated it was unstable protein. CsCCD7 expression in roots was the highest, while that in dwarf cucumber ‘D0462’ with lot of branches were the lowest by RT-PCR analysis. It suggested that the CsCCD7 protein could revolve in control branch signal transduction and related gene expression in cucumber.