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作 者 ：南芳茹;冯佳;谢树莲*

期 刊 ：植物研究 2014年 34卷 5期 页码：584-591

关键词：熊野藻属；UPA；系统发育；地理起源；

Keywords:Kumanoa, UPA, phylogenetic relationship, ancestral geographic analysis,

摘 要 ：以中国产的6种熊野藻属(Kumanoa)和串珠藻属(Batrachospermum)植物为材料，测定其UPA序列，并在GenBank中下载相关基因序列，通过贝叶斯法、最大似然法和邻接法构建系统发育树，分析其系统发育。结果显示，UPA序列核苷酸变异位点占序列长度的43.1%，其中简约信息位点占序列长度的31.1%，种间差异度为0~1.81%，属间差异度大于4.19%，可以作为分子标记用于系统发育分析。中国产熊野藻属的两个种和来自世界范围内的多数该属种类聚为一大支，支持该属的建立。基于UPA序列的地理起源分析显示，两属的祖先分布区节点主要在北美洲和大洋洲，然后向其它地区扩散，熊野藻属向外扩散后，在南美洲分布较为集中。

Abstract:The chloroplast UPA genes were amplified and sequenced for two species in genus Kumanoa from China, including K.intorta(former Batrachospermum intortum) and K.curvata(former B.curvatum), as well as another four Batrachospermum species. The sequences were aligned with related groups from GenBank. Phylogenetic trees were constructed using the methods of Bayesian analysis, maximum likelihood and neighbor-joining. The results showed that the percentages of nucleotide variation sites and parsimony informative sites in UPA are 43.1% and 31.1%, respectively. The species divergence within genus ranges 0-1.81% and between genus is greater than 4.19%, so it is potential to be a molecular barcode to analyze phylogenetic relationships. Two species of Kumanoa from China were clustered together with another species of this genus from across the world, and supported Kumanoa separated from Batrachospermum. The ancestral geographic analysis showed the genus Kumanoa was derived from Eurasia and North America, and then dispersed to other continents with modern geographic advantage in South America.

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