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作 者 ：吴翔宇;许志茹;曲春浦;李蔚;孙琦;刘关君*

期 刊 ：植物研究 2014年 34卷 1期 页码：37-43

关键词：毛果杨；NLP；基因家族；表达模式；

Keywords:Populus trichocarpa, NLP, gene family, expression pattern,

摘 要 ：NLP基因家族是一类特殊的转录因子，豆科植物根瘤的形成依赖于该基因家族的存在，在非豆科植物中具有调节植物硝酸盐吸收以及同化的功能。通过对毛果杨（Populus trichocarpa）基因组的生物信息学分析，共鉴定出14个毛果杨NLP基因家族成员，这些成员具有低亲水性的特点，基因结构保守，都含有RWP-RK以及PB1两个保守结构域。通过细胞定位预测，所有成员都定位在细胞核中。直系同源与旁系同源进化分析显示，NLP基因家族成员在漫长的进化过程中经历了严格的选择。染色体定位分析表明，毛果杨NLP基因家族成员坐落在毛果杨9条染色体之上，成员数量的扩增来自于杨柳科染色体自身的扩增事件。芯片数据分析结果显示，NLP基因家族成员在嫩叶，根和雄花中表达，部分基因在木质部以及种子萌发过程之中表达，但所有成员均不在成熟叶片中表达。

Abstract:NLP gene family is a special transcription factor, the initiation of nodule development is dependent on the function of this gene family, in other species plant it has the function of regulating plant nitrate absorption and assimilation. Genome-wide analysis in Populus trichocarpa had identified 14 NLP gene family members. These members have the characteristics of low hydrophilic, the conservative gene structure and contain RWP-RK and PB1 two conservative domain. The localization of all members of NLP genes in P.trichocarpa was predicted in the nucleus. Evolution analysis showed that NLP gene family members experience strict filter. Chromosome localization analysis showed that the members of P.trichocarpa NLP genes were located on 9 chromosomes, the expanding of NLPs in P.trichocarpa was caused by Salicaceae duplication events. Microarray data analysis showed that NLPs had a high transcript accumulation in leaf, root and male catkins, some genes were expressed in xylem, seed and female catkins, but no gene was detected in the mature leaf.

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