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作 者 ：刘伟1,2；李媛媛1,2；王洪政1,2*；杨磊1,2；祖元刚1,2

期 刊 ：植物研究 2015年 35卷 2期 页码：315-320

关键词：单叶省藤；总黄酮；超声辅助提取；响应面；抗氧化能力；

Keywords:Calamus simplicfoilius, flavonoids, ultrasound-assisted extraction, RSM, antioxidant activity,

摘 要 ：研究了单叶省藤（Calamus simplicfoilius）叶总黄酮的超声辅助提取工艺以及体外抗氧化活性。根据Box-Behnken试验设计的原理，选取乙醇浓度、料液比和提取时间3因素3水平进行试验，通过响应面分析得到优化的省藤叶总黄酮提取组合条件。研究结果表明，省藤叶总黄酮最佳超声辅助提取工艺条件为乙醇浓度72.7%，液料比22.5 mL·g^-1，提取时间30 min。在此条件下，总黄酮实际得率为19.67 mg·g^-1。与浸提和加热回流等传统提取方法相比，超声辅助提取在提取效率和能耗上具有明显优势。红外光谱分析表明3种提取方法获得的提取物均含有大量具有与芦丁类似结构的黄酮类物质。此外，单叶省藤叶总黄酮的DPPH自由基清除能力、还原能力、铁还原能力等方面稍低于维生素E，具有温和的体外抗氧化活性。而清除超氧阴离子能力明显高于维生素E。

Abstract:We studied the total flavonoids extraction from leaves of Calamus simplicfoilius using ultrasonic-assisted extraction technology. A 3-factor, 3-level central composite design involving the three independent variables followed by response surface analysis was performed for the further optimization of the extraction parameters. The optimal extraction conditions were 72.74% of ethanol volume fraction, 22.5 mL·g^-1 of liquid-solid ratio, and 30 min of extraction time. Under these conditions, the experimental extraction of total flavonoids yield was 19.67 mg·g^-1. Ultrasonic-assisted extraction could reach more flavonoids and less time and energy comparing with traditionally extraction methods including macerate extraction and heat reflux extraction. By IR spectrums, the extracts contained amounts of flavonoids which had similar structure with lutein. Additionally, the DPPH free radical scavenging activity, reducing power and iron reduction activity of total flavonoids extracted using ultrasonic-assisted technology were a little lower than vitamin E, and the superoxide anions scavenging activity was significantly higher than vitamin E.

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