Abstract:Helianthus annuus L. is one of the most famous ornamental plants and the oil crops in the world, its secondary metabolites have various biological and medical activities. We studied the distribution and accumulating regularity of four secondary metabolites-chlorogenic acid, polysaccharides, flavonoids and polyphenols in various growing stages of H.annuus L.. The higest chlorogenic acid content of 2.64 mg·g-1 was in faceplates on flowering stage, the highest polysaccharide content of 52.2 mg·g-1 was in faceplates on maturity stage, and the highest flavonoids and polyphenols contents of 20.94 and 11.75 mg·g-1, respectively, were in leaves on seedling stage. Considering the distribution and accumulating regularity of these four secondary metabolites, the faceplates on flowering stage and leaves on budding stage have the most important medicinal value in H.annuus.