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作 者 ：吴柏艳;谢彦梅;王振月*

期 刊 ：植物研究 2012年 32卷 6期 页码：685-688

关键词：毛脉酸模；组织培养；植株再生；

Keywords:Rumex gmelini Turcz, tissue culture, plant regeneration,

摘 要 ：以毛脉酸模下胚轴和幼苗根茎为外植体，以MS为基本培养基，与不同浓度比例的6-BA和2,4-D等植物生长调节剂进行毛脉酸模组织培养的研究。结果表明，毛脉酸模幼苗根茎是诱导丛生芽的最佳材料，诱导丛生芽产生的最佳培养基为MS+6-BA 3.0 mg·L^-1+2,4-D 0.1 mg·L^-1，诱导率为81.2%；生根培养基为无激素的MS培养基，生根率可达92%，在此条件下生根迅速，可获得完整植株；组培苗经过炼苗移栽后成活率可达80%。

Abstract:With the hypocotyls and seedling rhizomes of Rumex gmelini Turcz. as explants, the tissue culture of R.gmelini was studied by adding different concentrations of 6-BA and 2,4-D into MS basic mediums. The results showed that the better materials for multiple shoot clumps induction were seedling rhizomes; the best medium for multiple shoot clumps induction was MS+6-BA 3.0 mg·L^-1+2,4-D 0.1 mg·L^-1, the induction rate was 81.2%; the rooting medium was MS basic mediums without hormones, the rooting rate could get 92%, and under this conditions the plant regeneration could be obtained rapidly. After transplantation, about 80% of these seedlings could survive.

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