Abstract:The article explained the study on oil extraction with petroleum ether (60~90℃) from Xanthoceras sorbifolia Bunge. through single factor test and orthogonal test. It confirmed the optimum extraction condition were as follow: particle size 2 mm,extraction temperature 90℃,extraction time 10 h,ratio of solvent to seed 5:1(V/W). The average extraction rate under the optimum conditions was 62.49%. The composition of fatty acid in the oil was analyzed by HPLC. The results show that the component of the oil is mainly the same with biodiesel,and X. sorbifolia Bunge. seed oil will be a new resource of biodiesel production.