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作 者 ：郭艳茹;詹亚光*;纪丽丽

期 刊 ：植物研究 2007年 27卷 2期 页码：224-228

关键词：花楸腋芽；组织培养；快速繁殖；

Keywords:Sorbus pohuashanensis axillary bud, tissue culture, rapid propagation,

摘 要 ：选用花楸茎节为外植体进行组培腋芽增殖途径研究，结果表明：利于花楸生长的最适宜基本培养基为MS培养基；芽诱导培养基为1/2MS+6-BA 2.5 mg·L^-1+2.4-D 0.5 mg·L^-1；增殖培养基为MS+6-BA（1.600~2.100）mg·L^-1+NAA（0.140~0.230） mg·L^-1，平均繁殖系数达到13倍以上；继代壮苗培养的最佳培养基为MS+6-BA 0.2 mg·L^-1+IBA 0.2 mg·L^-1；在1/2MS+IBA 0.3 mg·L^-1培养基上，平均生根数为5.96，生根率达90.20%。将生根后的组培苗移栽至腐殖土：泥炭土：河沙（比例为3：2：1）的基质中，成活率达95.55%。

Abstract:In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L^-1) and 2.4-D(0.5 mg·L^-1); the optimum medium for bud proliferation was MS+6-BA(1 600~2 100)mg·L^-1+NAA(0.140~0.230)mg·L^-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L^-1+IBA0.2 mg·L^-1; And 1/2MS+IBA0.3 mg·L^-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

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