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作 者 ：井乐刚;张永忠*

期 刊 ：植物研究 2006年 27卷 5期 页码：629-632

关键词：大豆异黄酮；高效液相色谱；大豆乳清；

Keywords:soybean isoflavones, high performance liquid chromatography, soybean whey,

摘 要 ：建立了大豆乳清提取物中大豆异黄酮含量的高效液相色谱测定方法。采用Nova-Pak C₁₈（3.9×150 mm，4 μm）色谱柱；以甲醇：0.4%磷酸=30：70（v/v）为流动相分析染料木苷和黄豆苷；流速为0.7 mL·min^-1；柱温为30℃；检测波长为260 nm。试验结果表明，大豆乳清提取物中的大豆异黄酮含量为72.5%，其组成以染料木苷和黄豆苷为主，二者比例接近1∶1，苷元型大豆异黄酮未检出。染料木苷和黄豆苷的平均回收率分别为98.1%和98.4%，相对标准偏差（RSD）分别为0.7%（n=5）和0.8%(n=5)。该方法快速、准确、重复性好。

Abstract:A method for the determination of soybean isoflavones extracted from soybean whey by HPLC was established. Nova-Pak C₁₈ column (3.9×150 mm, 4 μm) was used. Genistin and daidzin were separated by mobile phase: methanol-0.4% phosphoric acid solution = 30:70 (v/v). The flow rate was 0.7 mL·min^-1. The column temperature was 30℃. The UV detection wavelength was 260 nm. The results showed that the content of soybean isoflavones in the soybean whey extract was 72.5%. Genistin and daidzin were the major components in the soybean isoflavones, and the ratio of the two components was nearly 1∶1. The isoflavone aglycons were not found by HPLC. The average recoveries of genistin and daidzin were 98.1% and 98.4% respectively, and the relative standard deviations of genistin and daidzin were 0.7% (n=5) and 0.8% (n=5) respectively. The method was rapid, accurate and with good reproducibility.

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