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作 者 ：柴民伟;石福臣*;马妍

期 刊 ：植物研究 2010年 30卷 6期 页码：758-762

关键词：药用植物；水华；防己；蛋白核小球藻；高效液相色谱；

Keywords:medicinal plant, water bloom, Stephania tetrandra, Chlorella pyrenoidosa, HPLC,

摘 要 ：通过常温水浸提方法获得12种药用植物浸提液，并分别研究其抑藻效应。结果显示：防己水浸提物(相对浓度1 g·L^-1)抑制蛋白核小球藻的效应最强，最大比生长率为-0.28 d^-1。防己甲醇浸提物对蛋白核小球藻的抑藻效应显著，且持续时间长，最低有效抑藻浓度为30 mg·L^-1。HPLC鉴定结果显示：防己水浸提液和甲醇浸提液中主要含有防己诺林碱、粉防己碱等生物碱类化学成分，防己对治理水华发生有较大的应用前景。

Abstract:Aqueous extracts of 12 kinds of medicinal plants were obtained by soak extraction, and their inhibitory effects on algae growth were studied. The results showed that: when the relative concentration was 1 g·L^-1, aqueous extracts of Stephania tetrandra had the strongest inhibitory effect on the growth of Chlorella pyrenoidosa, and the maximum specific growth rate was -0.28 d^-1. The methanol extract of S.tetrandra had significant and long-lasting inhibitory effect on the growth of C.pyrenoidosa and the lowest effective concentration in inhibiting algae was 30 mg·L^-1.The identification results of HPLC showed that: the main constituents in the extracts were alkaloids such as fangchinoline and tetrandrine, etc.S.tetrandra had great prospect of application in controlling water bloom.

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