---

作 者 ：甘霖;于肖夏;鞠天华;于卓*;蒙美莲;张自强;李长青

期 刊 ：植物研究 2013年 33卷 2期 页码：243-247

关键词：马铃薯；杂种F1；无性株系；ISSR鉴定；

Keywords:Potato, hybrids F1, clones, ISSR identification,

摘 要 ：为给马铃薯新品种选育提供可靠材料，采用ISSR分子标记对2个马铃薯杂交组合‘J07-4’ב陇薯6号’和‘J07-6’ב陇薯6号’杂交种F₁无性繁殖株系的真实性进行了鉴定。结果从152个ISSR引物中筛选出适于‘J07-4’ב陇薯6号’杂种F₁ 5个无性株系鉴定的3个ISSR引物AF18550、AW75511和AW20617及‘J076’ב陇薯6号’杂种F₁ 7个无性株系鉴定的2个ISSR引物AW20607和AF18549；以子代中含有父本特征带为主要依据，鉴定出杂种F₁共12个无性繁殖株系均为真实的杂交种，并建立了杂种F₁不同无性株系间的ISSR指纹图。表明ISSR分子标记技术用于马铃薯杂种F₁无性株系鉴定是可行的。

Abstract:In order to provide reliable materials for potato varieties breeding, the study was to identify the authenticity of clones of hybrids F₁ of two potato combinations, ‘J07-4’בLongshu No.6’ and ‘J07-6’בLongshu No.6’, using ISSR molecular markers. The results were that five primers were selected from 152 ISSR primers, primers AF18550, AW75511 and AW20617 were suitable to identify 5 clones of ‘J07-4’בLongshu No.6’ hybrids F₁, and AW20607 and AF18549 were suitable to identify 7 clones of ‘J07-6’בLongshu No.6’ hybrids F₁. A total of 12 clones were identified as true hybrids based on the paternal-specific bands in progeny’s bands. ISSR fingerprints of different clones of hybrids F₁ were established. It showed that it was feasible to use ISSR molecular markers to identify clones of potato hybrids F₁.

---