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作 者 ：石晋;栾雨时;*;王静云;田俊平

期 刊 ：植物研究 2008年 28卷 6期 页码：767-769

关键词：叶黄素；甘薯；HPLC；

Keywords:lutein, sweet potato, HPLC,

摘 要 ：建立甘薯叶片中有效成分叶黄素含量的高效液相色谱测定方法，以寻求含量高的甘薯品种。采用Waters SunFire^TM C₁₈(150 mm×4.6 mm,5 μm )色谱柱；以甲醇—水为95:5(V/V)为流动相，流速为0.8 mL·min^-1；检测波长为445 nm；外标法定量。此色谱条件下，叶黄素含量在5~100 μg·mL^-1范围内时，与峰面积呈线性关系；样品平均回收率为99.5%；相对标准偏差 (RSD)为1.9%(n=5)。该方法灵敏、准确、专属性强，适用于甘薯叶片中叶黄素的测定；大部分供试品种间存在显著差异，其中以苏薯8号的含量最高。

Abstract:A HPLC method for the determination of lutein in leaves of sweet potato was developed in this research for selecting the breed which has the highest content of lutein. The analysis was carried on a Waters SunFire^TM C₁₈(150 mm×4.6 mm,5 μm) column. The mobile phase was methanol-water (95:5, v/v), the flow-rate was 0.8 mL·min^-1 and wavelength was 445 nm. Linearity was found in the range of 5~100 μg·mL^-1, the average recovery was 99.5%, and the relative standard deviations for lutein was 1.9%(n=5). This method is reliable, accurate and suitable for the determination of lutein in leaves of sweet potato. There were significant differences in the lutein content of most samples tested and among them, Sushu No.8 are the highest.

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