全 文 ：第 22卷　第 3 期　　　　　　　　　　　　　植　　　物　　　研　　　究 2002 年　7月
Vol.22　No.3　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH July , 　2002
高效毛细管电泳法测定高山红景天中红景天甙含量的比较研究
谷会岩1 　宫立冬2　马　玲1
(1.东北林业大学 , 哈尔滨　150040)
(2.新林林业局 , 加格达奇　165023)
摘　要　采用高效毛细管电泳法对采集于两个野生产地不同居群和两个人工栽培地的高山红景
天中红景天甙的含量进行了对比研究 ,实验结果表明 ,该方法高效 、简便 、灵敏 、可靠;人工栽培高
山红景天和自然生境下高山红景天中红景天甙含量有差异 ,不同天然居群高山红景天中红景天甙
含量也有差异 。
关键词　红景天甙;高效毛细管电泳;高山红景天
A COMPARATIVE STUDY ON CONTENTS OF SALIDROSIDE
IN RHODIOLA SACHALINENSIS ROOT MEASURED
BY HIGH PERFORMANCE CAPILLAY ELECTROPHORESIS
GUHui-yan1　GONG Li-dong2　MA Ling1
(1.Northeast Forestry University , Harbin 150040)
(2.Xin Lin Forestry Bureau , Jiagedaqi 165023)
Abstract　The contents of salidroside in two populations of varied wild growing colonies and two samples of
cultivated root of Rhodiola sachalinensis at different places were measured by high performance capillary elec-
trophoresis comparatively.The results demonstrated that analytical method is of high performance , simple ,
sensitive , and reliable.The contents of salidroside in cultivated and wild growing roots of Rhodiola sachalin-
ensis present a difference , and the contents in different natural colonies show varied too.
Key words　salidroside;high performance capillary electrophoresis;Rhodiola sachalinensis A.Bor
1　Introduction
　　Rhodiola sachalinensis A.Bor is a perennial herb ,
Family:Crassalaceae.The plant is just only distributed at
Changbai Mountain area in Fusong and Antu Counties of
Jilin Province , and a part of the south-eastern margin of
Zhangguangcai Mountain area in Shangzhi and Hailin
Counties of Heilongjiang Province
[ 1] .The Rhodiola root
possesses of higher medicinal value being similar to gin-
seng performing an efficacy against senility and fatigue ,
Salidroside is a sort of active constituents of Rhodiola
species , which is recommended as a standard for assess-
ment of the quality of the root
[ 2 ,3] .The molecularweight of
salidroside is 300 , its chemical structural formula is
shown in Figure 1.In recent years , the researchers most-
ly employed HPLC method to measure the contents of sali-
droside in Rhodiola plants
[ 4 ～ 8] .In order to more accu-
rately and simply evaluate the quality of the natural and
cultivated roots of Rhodiola sachalinensis for the exploita-
第一作者简介:谷会岩(1970-),男,讲师 ,主要从事植物生态学研究。
收稿日期:2002-03-25
tion of native resource of the plant , this paper adopted a
high performance capillary electrophoresis for determining
the contents of salidroside collected from several produc-
tion places , meanwhile , the extraction means and the de-
termination condition for treating the samples were to be
compared , that was expected to confirm the quality of
Rhodiola roots and make a dependent basis for exploitati-
on and applications of the natural resource of Rhodiola
sachalinensis root.
Fig.1　The chemical structure of salidroside
2　Study method
2.1　Reagents and instruments:The standard sample of
salidroside was provided by Shenyang University of Phar-
macy , and a 270A-HT model high performance capillary
electrophoresis meter was US PE Company product.
Fig.2　The standard curve of salidroside
2.2 　Determination of the contents of salidroside:a)
Electrophoresis condition:capillary , 62cm×85μm (id);
checked wave , 280nm;separated electric pressure , 25
KV;entering electric pressure , 20mmHg;temperature ,
30℃.Used ultra-pure water and buffer solution to rinse
the capillary at each time before electrophoresis.Pre -
treatment of samples:employed 0.45μm water system mi-
cro -filter film to filtrate the buffer solution , standard
sample and tested samples individually , and entering the
samples for analysis immediately.b)Preparation of the
standard solution:Accurately weigh 5.4mg of salidroside
standard sample , added a small volume of 85% ethyl al-
cohol leading to dissolving , then place the solution into a
5ml volumetric bottle and adjusted the volume to the
scale , this solution was made as a mother -solution for
preparing the standard solutions of 0.54mg ml , 0.432mg
ml , 0.216mg ml , 0.108mg ml , 0.0864mg ml , 0.
032mg ml , and 0.0216mg ml respectively , each of one
share was waiting for analytic uses.c)Buffer solution:
Phosphoric acid buffer solution , 50mmol L , pH 5.8;d)
Drawing the standard curve:Utilized a micro-syringe to
draw the standard solutions accurately , entered the sam-
ples repeatedly three times according to the above-men-
Fig.3　The high performance capillary electrophoresis
picture of standard salidroside
tioned conditions , and took a n average value of peak area
each time , the values were measured as:2088310 ,
1063368 , 855974 , 758472 , 546314 , 487325 , 365514 ,
and 212507;using the peak area values of the former five
values as the ordinate and the contents of salidroside as
the abscissa , the linear regression equation was obtained
as the following:Y=3.19105×105+1.564356×106X ,
the relation coefficient , r =0.9928.The experimental
demonstrated that there is a better linear correlation ship
between the standard solution within a range of 0.108mg
ml to 1.08mg mml and the peak area value , meanwhile ,
the effect of the isolation of salidroside was better , as
shown in Figure 3.
338 　　　　　　植　　物　　研　　究　　　　　　　　　　　　　　　　　　22 卷
2.3　Determination of the contents of salidroside in vari-
ous samples of Rhodioila sachalinensis roots:a)Prepara-
tion of sample solutions:Placed the raw materials of the
samples to heat (80℃)to dryness of a constant weight
and sifted through a sieve of 60 meshes , wrapped with fil-
ter paper to place into a Soxhlet apparatus , added 85%
ethyl alcohol to extract on a water-bath (90℃)for 4
hours , collected the filtrate to put into a volumetric bottle
adjusting the volume to 70ml fro use of quantitative analy-
sis.b)Determination on contents of salidroside:Took the
sample solutions of Rhodiola sachalinensis root according
to the condition for drawing the standard curve to deter-
mine the contents of salidroside five times repeatedly ,
used the mean value of the five measured values of the
peak area , depending upon the standard curve equation
calculated out the contents of salidroside , see Table 1.c)
Rate of recovery:Eight powered samples of Rhodiola root
were weighed up accurately and 0.3mg of standard sali-
droside was added to each sample , the Rhodiola sample
was determined three times by the above -mentioned
method , and the mean value was used to calculate the
recovery rate and a 97.7% recovery was obtained , this
indicated the foregoing method possesses of a higher accu-
racy .
3　Results and Discussion.
3.1　To employ the high performance capillary electro-
phoresis for the measurement of the content of salidroside
in Rhodiola sachalinensis root is unnecessary to recom-
mend a special treatment for the samples , the determina-
tion offers merits of high performance , simple operation ,
and better sensitivity , and the method is quite to be reli-
able.
3.2　The results expressed that compared between the
wild growing and cultivated Rhodiola sachalinensis roots
the contents of salidroside displayed a dominant differ-
ence;while in different area , the wild growing Rhodiola
sachalinensis roots also presented a varied contents of sali-
droside , and at different places , the cultivated roots gave
a certain variance , but it was indistinct between them.
This demonstrated the content of the secondary metabolic
product , salidroside , is affected by the condition of cir-
cumstances
[ 9 ～ 11]
Table 1　The contents of salidroside in different wild growing and cultivated Rhodiola
No. Rhodiola species Used organ part Natural growing area cultivating place Salidroside content(%)
1 Rh.Sachalinensis root Hailin , Heilongjiang (natural growing) 1.04
2 Rh.Sachalinensis root Songjianghe , Jilin(natural growing) 0.53
3 Rh.Sachalinensis root Changbai Mountain , Wenquan(natural growing) 0.40
4 Rh.Sachalinensis root Changbai Mountain , Tianwenfeng (natural growing) 1.13
5 Rh.Sachalinensis root Daxing anling , Heilongjiang(cultivated) 0.505
6 Rh.Sachalinensis root Hailin , Heilongjiang(cultivated) 0.611
　　
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