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作 者 ：王禾;冯玉兰;黄笛;董丽;

期 刊 ：植物研究 2012年 32卷 (3)期 页码：270-274

关键词：粗茎鳞毛蕨；离心；消毒；培养基；光质；孢子萌发；

Keywords:Dryopteris crassirhizoma, centrifugal, disinfection, culture media, light quality, spore germination,

摘 要 ：以经过3年低温储藏的粗茎鳞毛蕨孢子为实验材料，从孢子离心、孢子消毒、培养基种类、光质等4方面对孢子萌发进行研究，结果表明: 在离心转数≤14 000 r·min^-1、离心时间≤30 min条件下，离心处理对孢子萌发基本无影响；对孢子进行1% NaClO水溶液浸泡处理20~30 min为最佳消毒条件；改良Knop’s培养基为最佳孢子萌发培养基；黑暗条件下孢子不能萌发，但是黑暗处理能够明显提高孢子萌发整齐性；红光比白光能促进孢子提早萌发1 d左右，但对提高萌发率效果不显著。

Abstract:In this paper, the spores of Dryopteris crassirhizoma after three years of low-temperature storage were used as the experimental material, the influences of centrifugal, disinfection, culture media and light quality on spore germination were respectively studied. The results showed that centrifugal (≤14 000 r·min^-1, ≤30 min) almost had no influence on spore germination; Sterilizing spore surface for 10-20 min with 1% NaClO was the best sterile method; Modified Knop’s medium was the best for spore germination; Spore could not germinate in dark, but the dark treatment could improve the regularity of spore germination; Red light could promote spore germinating about 1 day earlier than white light, but had no obvious influence on promoting spore germination rate.

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