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作 者 ：李田;王逸群;陆兆华*

期 刊 ：植物研究 2009年 29卷 4期 页码：460-465

关键词：乙肝表面抗原基因；表达载体；烟草；植物疫苗；

Keywords:hepatitis B surface antigen gene, expression vector, tobacco, plant vaccine,

摘 要 ：构建了植物表达载体pBRSAg，该载体具有完整的植物表达元件，CaMV35S启动子、农杆菌T-DNA左右边界、植物报告基因gus和植物选择标记基因hpt，适用于农杆菌的转化；通过冻融法将重组质粒pBRSAg转入根癌农杆菌LBA4404中，利用农杆菌介导法转化烟草叶盘，经筛选培养获得烟草植株。抗性植株经GUS染色和PCR检测为阳性，初步表明乙肝表面抗原基因在烟草中得到表达。

Abstract:The plant expression vector pBRSAg was constructed, which contained all elements for plant expression, such as CaMV35S promoter, both left and right border sequence for T-DNA in Agrobacteria, plant reporter gene gus, and plant screening marker gene hpt, and was suitable for transformation via Agrobacteria. The recombinant binary vector pBRSAg was mobilized into Agrobacterium tumefaciens strain LBA4404 cells by using the freeze-thaw method. Tobacco leaf discs were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining and PCR test of the plants were positive. It was initially shown that the HBsAg gene was expressed in transgenic tobacco plants.

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