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作 者 ：王亚楠;冯佳;谢树莲*

期 刊 ：植物研究 2011年 31卷 (3)期 页码：257-260

关键词：psaA；psbA；红索藻目；系统发育；

Keywords:psaA, psbA, Thoreales, phylogenetic relationship,

摘 要 ：对红索藻目植物棘刺红索藻(Thorea hispida)的psaA和psbA基因进行扩增和测序，并与其它类群比对分析，分别用最大简约法、邻接法和贝叶斯法构建系统发育树。结果显示，psaA和psbA基因测得的序列片段分别为825和920 bp，psaA基因A、T、C、G碱基含量分别为29.9%、35.8%、16.5%和17.8%，psbA基因A、T、C、G碱基含量分别为27.5%、35.3%、16.8%和20.4%，两个基因A+T含量均高于C+G含量，说明两个基因在进化上均有碱基的偏好性。用3种方法所构建的系统树拓扑结构基本一致，红索藻目植物均聚合于一个分支，独立于其它类群，支持红索藻目为一独立的目。

Abstract:Two genes, psaA and psbA, were amplified and sequenced for Thorea hispida(Thoreales) and blasted with other groups, then the phylogenetic trees based on two genes were generated by the methods of Neighbor-Joining, Maximum Parsimony and Bayesian. The results showed the sequences of psaA and psbA were 825 bp and 920 bp in length, respectively. A, T, C and G contents of psaA were 29.9%, 35.8%, 16.5% and 17.8% and those of psbA were 27.5%, 35.3%, 16.8% and 20.4%. There was higher content of A+T than that of G+C for two genes. It indicated that these two genes had favor in nucleotide. The phylogenetic trees generated from three methods displayed the similar topologic structures and the species of Thoreales were aggregated in one branch, separated from other groups. This result supported that Thoreales was an independent order.

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