Abstract:Colonization trends of endophytic bacteria EBS05 in wheat was assayed using the antibiotic resistant mutation strains as screening marker using the method of spread-plate technique, and its control effect on wheat sharp eyespot was investigated using pot culture and field test. The results showed that the endophytic bacteria EBS05 exhibited strong colonization capacity in wheat, and could move from root to stem and/or leaf. The colonization amount was in direct proportion to inoculation concentration, only which was more than 108 CFU/mL, the colonization and further transfer could effectively occurred. After treatment by soaking seed and drench with fermentation broth at 108 CFU/mL cell density, colonization amount of strain EBS05 in wheat root was more than that in stem and leaf at the same treatment time. The colonization amounts in root, stem and leaf increased gradually in the early stage of treatment, reached peaks at the tenth or twelfth day after treatment, and decreased gradually after that time. Application of the culture broth of endophytic bacteria EBS05 was proved to be more effective in controlling wheat sharp eyespot, compared with that of fungicide fludioxonil. The suppressive effects of fermentation broth and bacteria-free filter on the wheat sharp eyespot were up to 91.2% and 88.2% in the greenhouse, and 66.3% and 56.2% in the field, respectively.