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期 刊 ：植物保护学报 2005年

关键词：小麦纹枯病菌；分子检测；

Keywords:Rhizoctonia cerealis, molecular detecting,

摘 要 ：小麦茎基部可寄居多种真菌,并可导致发褐、坏死等症状,与小麦纹枯病相混淆。为准确诊断小麦纹枯病,根据江苏省小麦茎基部常见寄居菌禾谷丝核菌Rhizoctonia cerealis、立枯丝核菌Rhizoctonia solani、长蠕孢菌Helminthosporium spp.、交链孢菌Alternaria spp.、小麦全蚀病菌Gaeumannomyces graminis var. tritici 和禾谷镰刀菌Fsarium graminearum rDNA的ITS区段序列差别,设计合成了2对特异性扩增引物(DNF77+DNR554,DNF81+DNR564)用于小麦纹枯病菌检测。2对引物均能从小麦纹枯病菌株扩增到特异性的分子片段,说明设计的特异性引物可以用来检测小麦纹枯病菌。采集田间具有小麦纹枯病症状、茎基部变褐及健康麦苗,进行病原菌分离,同时提取这些麦苗茎基部DNA,利用上述2对引物进行扩增。结果表明,仅从能分离到禾谷丝核菌的麦组织中扩增到约480bp的特异性条带。说明设计的特异性引物可以对田间小麦纹枯病进行早期、快速分子诊断。

Abstract:According to the difference of rDNA ITS sequence among Rhizoctonia cerealis, Rhizoctonia solani, Helminthosporium spp., Alternaria spp., Gaeumannomyces graminis var. tritici and Fusarium graminearum, which usually inhabits bases of wheat plants, two pairs of primers, DNF77 +DNR554 and DNF81+DNR564, were designed to detect Rhizoctonia cerealis which cause wheat sharp eyespot. The results showed that a sequence about 480bp could be amplified by PCR from pure cultured R.cerealis isolates and infected sheath of wheat by R.cerealis isolates using the designed primers, which could be used to develop a rapid PCR-based diagnostic technique for the sharp eyespot at early stage of R.cerealis infecting wheat.

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