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期 刊 ：植物保护学报 2005年

关键词：玉米纹枯病；拮抗细菌；促生作用；内生性检测；生物防治；

Keywords:Corn sheath blight, antagonistic bacteria, promotion effect, endophytic detection, biological control,

摘 要 ：从玉米成株期根、茎组织中分离获得232株内生细菌,在离体条件下筛选获得20株对玉米纹枯病菌具有显著拮抗作用的内生细菌,其中7株为枯草芽孢杆菌,占拮抗菌株的35%。B20-120、B20-006、B20-122、B21-072、B21-016、B20-070等6株拮抗细菌的发酵液对玉米种子萌发没有影响,进一步对其内生性(以抗利福平120 mg·mL^-1为标记)及其对玉米的促生作用和玉米纹枯病的防治效果进行了研究。结果表明,供试6个拮抗菌株对玉米生长没有抑制作用,有的甚至有促进作用,并且能在体内繁殖,具可转移性。拮抗菌株B20-006和B20-120对纹枯病的防治效果最好,菌液浸种处理苗期防治效果分别为67.9%和62.3%,成株期分别为40.2%和39.1%。

Abstract:Two hundred and thirty-two strains of endophytic bacteria were isolated from corn root,stem tissues during adult period.By way of in vitro screening,20 antagonistic strains against Rhizoctonia solani (7 strains Bacillus subtilis,about 35% of the total antagonistic strains screened) were gotten.Among the 20 strains,6 strains (B20-120,B20-006,B20-122,B21-072,B21-016,B20-070) had been proved that their fermented solution did not inhibit corn seed germination.Endophytic detection (tagged by the resistance to Rif,120 mg·mL^-1) of the 6 strains,their growth-promoting effects on corn, and control effects against corn sheath blight were studied.The results showed that these strains could live,and transfer in corn plant,while the growth-promoting effect of the strains on corn was evident.Two strains,B20-006 and B20-120,were efficient biological control agents to control corn sheath blight.Seed coating with 2 strains cultures gave 67.9% and 62.3% control efficacies at seedling stage,40.2% and 39.1% at adult stage,respectively.

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