Abstract:The effect of propamidine on growth and development of Botrytis cinerea Pers. on living plants were examined using electron microscopy techniques. The studied revealed that propamidine have distinct influence on growth and development of Botrytis cinerea Pers., but there were some difference when sprayed propamidine at various periods. Scanning electron micrographs revealed that the quantity of conidium decline and conidia collapsed practice of propamidine at the beginning of infestation; don’t influence the quantity of conidium but conidia collapsed practice of propamidine after infestation. Transmission electron micrographs revealed that Botrytis cinerea Pers. can’t invade tomato of epidermal organise practice of propamidine at the beginning of infestation; but Botrytis cinerea Pers. can invade tomato of epi- dermal organise collapsed practice of propamidine after infestation. Propamidine can inhibited the hyphal growth, the tip of the hyphae were shrunken, considerable thickening of the hyphal cell walls, particularly at the hyphal tip region, cytoplasm inhomogeneity and inclusion in cell exosmosis, mitochondrion manifold, vesiculated hypha cell, cell empty and so on when sprayed propamidine at various periods. The cytological change resulted in the necrosis of hyphal cell. The studied showed that propamidine had distinct protective and therapeutic action influence.