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期 刊 ：植物保护学报 2006年

关键词：苏云金芽孢杆菌；HBF-1菌株；金龟科；花生；草坪；

Keywords:Bacillus thuringiensis, HBF-1 strain, Scarabaeoidae, peanut, lawn,

摘 要 ：以我国筛选出的对金龟科幼虫具有杀虫活性的HBF-1菌株为供试菌株,采用菌悬液与含有土豆丝的土壤混合饲喂幼虫的方法,测定了HBF-1菌株孢晶粉剂对华北地区主要金龟子幼虫的毒力,其敏感性依次为四纹丽金龟Popillia quadriguttata、黄褐丽金龟Anomala exoleta、蒙古丽金龟A. mongolica、铜绿丽金龟A. corpulenta和苹毛丽金龟Proagopertha lucidula,LC₅₀为3.104、18.987、30.924、34.713和55.995 μg/g土。HBF-1菌株悬浮剂防治花生田金龟子幼虫的田间小区试验结果表明,50倍液的虫口减退率为71.4%~75.4%,保果效果为58.7%~72.9%。在花生产量比较试验中,HBF-1菌株悬浮剂50倍液处理区比空白对照区的产量增加363.0~903.0 kg/hm²。HBF-1菌株悬浮剂施入草坪后可免受阳光中紫外线的照射,杀虫活性保持较长时间;经调查,HBF-1制剂在土壤中经过120 天后仍保持杀虫活性。

Abstract:To test the toxicity of spore-crystal formulation of HBF-1 specific to larvae of several main scarabaeid species in North China, Bacillus thuringiensis suspensions mixed with sterilized fine soil containing potato pieces were used for feeding the larvae. The LC₅₀ of HBF-1 crystal-spore powder to Popillia quadriguttata, Anomala exoleta, A.mongolica, A.corpulenta and  Proagopertha lucidula were 3.104, 18.987, 30.924, 34.713 and 55.995 μg/g soil, respectively. Among them, P.quadriguttata was most sensitive to HBF-1. Plot experiment was conducted to control scarab larvae using HBF-1 SC in peanut field. The number of larvae decreased by 71.4%-75.4% at the 50 times dilution, and its nut-retention rate reached to 58.7%-72.9%, and the yield of the nut at 50 times dilution increased by 363.0-903.0 kg/hm² compared to that of the control. After application, the insecticidal activity of HBF-1 in the lawn can persist for a longer period due to avoiding of ultraviolet irradiation, and its activity can keep 120 days after application.

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