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作 者 ：许良政;张福锁;李春俭

期 刊 ：植物营养与肥料学报 1998年 4卷 1期 页码：63-66

关键词：2rdquo；mdash；联吡啶比色法；双子叶植物；Fe(Ⅲ)mdash；还原酶活性；

摘 要 ：对影响2，2’—联吡啶牌比色法测定双子叶植物根细胞原生质膜上Fe³⁺还原酶活性的有关因素进行了比较研究，确定了适宜的测试条件。结果表明，2，2’—联吡啶比色法测定的最大吸收波长为253.3nm，根系与反应液的适宜反应时间为2小时。反应过程中是否通气以及反应后放置时间均不影响最大吸收峰处的测定值。与黑暗相比，在光照下进行反应可明显提高还原酶活性。

Abstract:The effects of several key factors on measuring Fe³⁺ reductase activity in Roots of dicotyledonous plants using 2,2‘-bipyridyl-colorlmetric method were studied in nutrient solution culture under the controlled conditions. The results showed that the highest peak of absorption spectrum was 253. 3nm, the appropriate reaction time of roots in reactive solution was 2 hours. In comparison with darkness the Fe³⁺ reductase activity was increased significantly under light during the reaction. But aeration during the reaction (2hr.) and either immediate or still stay of reactive solution for different times (0,3.6,16hr.) after the reaction until colorimetric analysis didn‘t irifluence the final measuring results.

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